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10.3390/ijms22052723 http://scihub22266oqcxt.onion/10.3390/ijms22052723 33800363!7962843!33800363     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2021 ; 22 (5): ä Nephropedia Template TP {{tp|p=33800363|t=2021. Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19 |pdf=|usr=}}{{33800363}} gab.com Text Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19 JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33800363 #FOAvip Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow cytometry-based serological and neutralization assays. The serology assays test the IgG and IgM against both the full-length spike antigens and the receptor binding domain (RBD) of the spike antigen. Benchmarking against an RBD-specific SARS-CoV IgG reference standard, the anti-SARS-CoV-2 RBD antibody titer was quantified in the range of 37.6 microg/mL to 31.0 ng/mL. The quantitative assays are highly specific with no correlative cross-reactivity with the spike proteins of MERS, SARS1, OC43 and HKU1 viruses. We further demonstrated good correlation between anti-RBD antibody titers and neutralizing antibody titers. The suite of serology and neutralization assays help to improve measurement confidence and are complementary and foundational for clinical and epidemiologic studies. Twit Text FOAVip Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19 ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33800363 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33800363 #FOAvip English Wikipedia <ref>{{Cite pmid|33800363}}</ref> Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19 #MMPMID33800363 Tian L; Elsheikh EB; Patrone PN; Kearsley AJ; Gaigalas AK; Inwood S; Lin-Gibson S; Esposito D; Wang L Int J Mol Sci 2021[Mar]; 22 (5): ä PMID33800363show ga Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow cytometry-based serological and neutralization assays. The serology assays test the IgG and IgM against both the full-length spike antigens and the receptor binding domain (RBD) of the spike antigen. Benchmarking against an RBD-specific SARS-CoV IgG reference standard, the anti-SARS-CoV-2 RBD antibody titer was quantified in the range of 37.6 microg/mL to 31.0 ng/mL. The quantitative assays are highly specific with no correlative cross-reactivity with the spike proteins of MERS, SARS1, OC43 and HKU1 viruses. We further demonstrated good correlation between anti-RBD antibody titers and neutralizing antibody titers. The suite of serology and neutralization assays help to improve measurement confidence and are complementary and foundational for clinical and epidemiologic studies. |Antibodies, Neutralizing/blood/immunology[MESH] |Antibodies, Viral/blood/immunology[MESH] |COVID-19 Serological Testing/*methods/*standards[MESH] |COVID-19/*blood/*immunology[MESH] |Cross Reactions[MESH] |Flow Cytometry/methods[MESH] |Fluorescence[MESH] |Humans[MESH] |Immunoglobulin G/blood/immunology[MESH] |Immunoglobulin M/blood/immunology[MESH] |Microspheres[MESH] |Neutralization Tests/*methods/*standards[MESH] |Receptors, Virus/chemistry/immunology[MESH] |SARS-CoV-2/*immunology[MESH]Towards Quantitative and Standardized Serological and Neutralization A(epmc).pdf DeepDyve Pubget Overpricing