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10.1016/j.jim.2021.113044 http://scihub22266oqcxt.onion/10.1016/j.jim.2021.113044 33785349!7999798!33785349     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol+Methods 2021 ; 494 (ä): 113044 Nephropedia Template TP {{tp|p=33785349|t=2021. SARS-CoV-2 diagnostics: Towards a more comprehensive approach to routine patient testing |pdf=|usr=}}{{33785349}} gab.com Text SARS-CoV-2 diagnostics: Towards a more comprehensive approach to routine patient testing JO: J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33785349 #FOAvip The SARS-CoV-2 pandemic has provided the stimulus for the rapid development of a variety of diagnostic testing methods. Initially these were deployed as screening tools to evidence spread of the virus within populations. The recent availability of vaccines against the virus and the need to better understand the parameters of post-infection protective immunity requires development of methods, suitable for use in the routine diagnostic laboratory, capable of characterising the viral immune response in greater detail. Such methods need to consider both cellular and humoral immunity. Toward this aim we have investigated use of a commercial multiplex assay (COVID Plus Assay, One Lambda), providing assessment of the SARS-CoV-2 response at structural level, and developed an in-house cell stimulation assay using commercially available viral peptides (Miltenyi). This paper reports our experience in use of these methods in extended investigation of a cohort of healthcare workers with prior screening results indicative of viral infection. The antibody response generated is shown to be both qualitatively and quantitatively different in different individuals. Similarly a recall response to SARS-CoV-2 antigen involving the T cell compartment can be readily demonstrated in recovered individuals but is of variable magnitude. Twit Text FOAVip SARS-CoV-2 diagnostics: Towards a more comprehensive approach to routine patient testing ????J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33785349 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33785349 #FOAvip English Wikipedia <ref>{{Cite pmid|33785349}}</ref> SARS-CoV-2 diagnostics: Towards a more comprehensive approach to routine patient testing #MMPMID33785349 Carter C; Hughes P; McHugh A; Nadat F; Lewthwaite P; Savic S; Clark B J Immunol Methods 2021[Jul]; 494 (ä): 113044 PMID33785349show ga The SARS-CoV-2 pandemic has provided the stimulus for the rapid development of a variety of diagnostic testing methods. Initially these were deployed as screening tools to evidence spread of the virus within populations. The recent availability of vaccines against the virus and the need to better understand the parameters of post-infection protective immunity requires development of methods, suitable for use in the routine diagnostic laboratory, capable of characterising the viral immune response in greater detail. Such methods need to consider both cellular and humoral immunity. Toward this aim we have investigated use of a commercial multiplex assay (COVID Plus Assay, One Lambda), providing assessment of the SARS-CoV-2 response at structural level, and developed an in-house cell stimulation assay using commercially available viral peptides (Miltenyi). This paper reports our experience in use of these methods in extended investigation of a cohort of healthcare workers with prior screening results indicative of viral infection. The antibody response generated is shown to be both qualitatively and quantitatively different in different individuals. Similarly a recall response to SARS-CoV-2 antigen involving the T cell compartment can be readily demonstrated in recovered individuals but is of variable magnitude. |*COVID-19 Serological Testing[MESH] |*COVID-19/diagnosis/epidemiology/immunology[MESH] |*Immunity, Cellular[MESH] |*Immunity, Humoral[MESH] |*Pandemics[MESH] |Antigens, Viral/chemistry/immunology[MESH] |Humans[MESH] |Peptides/chemistry/immunology[MESH] |SARS-CoV-2/*immunology[MESH]SARS-CoV-2 diagnostics: Towards a more comprehensive approach to routi(epmc).pdf DeepDyve Pubget Overpricing