Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.antiviral.2021.105064 http://scihub22266oqcxt.onion/10.1016/j.antiviral.2021.105064 33781803!7997689!33781803     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Antiviral+Res 2021 ; 190 (ä): 105064 Nephropedia Template TP {{tp|p=33781803|t=2021. Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection |pdf=|usr=}}{{33781803}} gab.com Text Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection JO: Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=33781803 #FOAvip COVID-19 is currently a highly pressing health threat and therapeutic strategies to mitigate the infection impact are urgently needed. Characterization of the SARS-CoV-2 interactome in infected cells may represent a powerful tool to identify cellular proteins hijacked by viruses for their life cycle and develop host-oriented antiviral therapeutics. Here we report the proteomic characterization of host proteins interacting with SARS-CoV-2 Nucleoprotein in infected Vero E6 cells. We identified 24 high-confidence proteins mainly playing a role in RNA metabolism and translation, including RNA helicases and scaffold proteins involved in the formation of stress granules, cytoplasmic aggregates of messenger ribonucleoproteins that accumulate as a result of stress-induced translation arrest. Analysis of stress granules upon SARS-CoV-2 infection showed that these structures are not induced in infected cells, neither eIF2alpha phosphorylation, an upstream event leading to stress-induced translation inhibition. Notably, we found that G3BP1, a stress granule component that associates with the Nucleoprotein, is required for efficient SARS-CoV-2 replication. Moreover, we showed that the Nucleoprotein-interacting RNA helicase DDX3X colocalizes with viral RNA foci and its inhibition by small molecules or small interfering RNAs significantly reduces viral replication. Altogether, these results indicate that SARS-CoV-2 subverts the stress granule machinery and exploits G3BP1 and DDX3X for its replication cycle, offering groundwork for future development of host-directed therapies. Twit Text FOAVip Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection ????Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=33781803 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33781803 #FOAvip English Wikipedia <ref>{{Cite pmid|33781803}}</ref> Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection #MMPMID33781803 Ciccosanti F; Di Rienzo M; Romagnoli A; Colavita F; Refolo G; Castilletti C; Agrati C; Brai A; Manetti F; Botta L; Capobianchi MR; Ippolito G; Piacentini M; Fimia GM Antiviral Res 2021[Jun]; 190 (ä): 105064 PMID33781803show ga COVID-19 is currently a highly pressing health threat and therapeutic strategies to mitigate the infection impact are urgently needed. Characterization of the SARS-CoV-2 interactome in infected cells may represent a powerful tool to identify cellular proteins hijacked by viruses for their life cycle and develop host-oriented antiviral therapeutics. Here we report the proteomic characterization of host proteins interacting with SARS-CoV-2 Nucleoprotein in infected Vero E6 cells. We identified 24 high-confidence proteins mainly playing a role in RNA metabolism and translation, including RNA helicases and scaffold proteins involved in the formation of stress granules, cytoplasmic aggregates of messenger ribonucleoproteins that accumulate as a result of stress-induced translation arrest. Analysis of stress granules upon SARS-CoV-2 infection showed that these structures are not induced in infected cells, neither eIF2alpha phosphorylation, an upstream event leading to stress-induced translation inhibition. Notably, we found that G3BP1, a stress granule component that associates with the Nucleoprotein, is required for efficient SARS-CoV-2 replication. Moreover, we showed that the Nucleoprotein-interacting RNA helicase DDX3X colocalizes with viral RNA foci and its inhibition by small molecules or small interfering RNAs significantly reduces viral replication. Altogether, these results indicate that SARS-CoV-2 subverts the stress granule machinery and exploits G3BP1 and DDX3X for its replication cycle, offering groundwork for future development of host-directed therapies. |*COVID-19 Drug Treatment[MESH] |Animals[MESH] |Antiviral Agents/*pharmacology[MESH] |COVID-19/*metabolism/virology[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |DEAD-box RNA Helicases/*metabolism[MESH] |DNA Helicases[MESH] |Eukaryotic Initiation Factor-2/metabolism[MESH] |Host-Pathogen Interactions[MESH] |Humans[MESH] |Poly-ADP-Ribose Binding Proteins/genetics/metabolism[MESH] |Proteomics/methods[MESH] |RNA Helicases[MESH] |RNA Recognition Motif Proteins/metabolism[MESH] |RNA, Small Interfering/metabolism[MESH] |RNA, Viral/metabolism[MESH] |SARS-CoV-2/metabolism[MESH] |Vero Cells[MESH]Proteomic analysis identifies the RNA helicase DDX3X as a host target (epmc).pdf DeepDyve Pubget Overpricing