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10.1016/j.bios.2021.113168 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113168 33780853!7970423!33780853     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 182 (ä): 113168 Nephropedia Template TP {{tp|p=33780853|t=2021. Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests |pdf=|usr=}}{{33780853}} gab.com Text Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33780853 #FOAvip We report an electrochemical biosensor combined with recombinase polymerase amplification (RPA) for rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2. The electrochemical biosensor based on a multi-microelectrode array allows the detection of multiple target genes by differential pulse voltammetry. The RPA reaction involves hybridization of the RPA amplicon with thiol-modified primers immobilized on the working electrodes, which leads to a reduction of current density as amplicons accumulate. The assay results in shorter "sample-to-answer" times than conventional PCR without expensive thermo-cycling equipment. The limits of detection are about 0.972 fg/muL (RdRP gene) and 3.925 fg/muL (N gene), which are slightly lower than or comparable to that of RPA assay results obtained by gel electrophoresis without post-amplification purification. The combination of electrochemical biosensors and the RPA assay is a rapid, sensitive, and convenient platform that can be potentially used as a point-of-care test for the diagnosis of COVID-19. Twit Text FOAVip Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33780853 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33780853 #FOAvip English Wikipedia <ref>{{Cite pmid|33780853}}</ref> Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests #MMPMID33780853 Kim HE; Schuck A; Lee SH; Lee Y; Kang M; Kim YS Biosens Bioelectron 2021[Jun]; 182 (ä): 113168 PMID33780853show ga We report an electrochemical biosensor combined with recombinase polymerase amplification (RPA) for rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2. The electrochemical biosensor based on a multi-microelectrode array allows the detection of multiple target genes by differential pulse voltammetry. The RPA reaction involves hybridization of the RPA amplicon with thiol-modified primers immobilized on the working electrodes, which leads to a reduction of current density as amplicons accumulate. The assay results in shorter "sample-to-answer" times than conventional PCR without expensive thermo-cycling equipment. The limits of detection are about 0.972 fg/muL (RdRP gene) and 3.925 fg/muL (N gene), which are slightly lower than or comparable to that of RPA assay results obtained by gel electrophoresis without post-amplification purification. The combination of electrochemical biosensors and the RPA assay is a rapid, sensitive, and convenient platform that can be potentially used as a point-of-care test for the diagnosis of COVID-19. |*Biosensing Techniques[MESH] |*Nucleic Acid Amplification Techniques[MESH] |*Point-of-Care Testing[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |SARS-CoV-2/isolation & purification[MESH]Sensitive electrochemical biosensor combined with isothermal amplifica(epmc).pdf DeepDyve Pubget Overpricing