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10.7554/eLife.65113 http://scihub22266oqcxt.onion/10.7554/eLife.65113 33779548!8057811!33779548     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Elife 2021 ; 10 (ä): ä Nephropedia Template TP {{tp|p=33779548|t=2021. Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers |pdf=|usr=}}{{33779548}} gab.com Text Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=33779548 #FOAvip Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The test has two steps: (1) heat saliva with a stabilization solution and (2) detect virus by incubating with a primer/enzyme mix. After incubation, saliva samples containing the SARS-CoV-2 genome turn bright yellow. Because this test is pH dependent, it can react falsely to some naturally acidic saliva samples. We report unique saliva stabilization protocols that rendered 295 healthy saliva samples compatible with the test, producing zero false positives. We also evaluated the test on 278 saliva samples from individuals who were infected with SARS-CoV-2 but had no symptoms at the time of saliva collection, and from 54 matched pairs of saliva and anterior nasal samples from infected individuals. The Saliva TwoStep test described herein identified infections with 94% sensitivity and >99% specificity in individuals with sub-clinical (asymptomatic or pre-symptomatic) infections. Twit Text FOAVip Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=33779548 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33779548 #FOAvip English Wikipedia <ref>{{Cite pmid|33779548}}</ref> Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers #MMPMID33779548 Yang Q; Meyerson NR; Clark SK; Paige CL; Fattor WT; Gilchrist AR; Barbachano-Guerrero A; Healy BG; Worden-Sapper ER; Wu SS; Muhlrad D; Decker CJ; Saldi TK; Lasda E; Gonzales P; Fink MR; Tat KL; Hager CR; Davis JC; Ozeroff CD; Brisson GR; McQueen MB; Leinwand LA; Parker R; Sawyer SL Elife 2021[Mar]; 10 (ä): ä PMID33779548show ga Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The test has two steps: (1) heat saliva with a stabilization solution and (2) detect virus by incubating with a primer/enzyme mix. After incubation, saliva samples containing the SARS-CoV-2 genome turn bright yellow. Because this test is pH dependent, it can react falsely to some naturally acidic saliva samples. We report unique saliva stabilization protocols that rendered 295 healthy saliva samples compatible with the test, producing zero false positives. We also evaluated the test on 278 saliva samples from individuals who were infected with SARS-CoV-2 but had no symptoms at the time of saliva collection, and from 54 matched pairs of saliva and anterior nasal samples from infected individuals. The Saliva TwoStep test described herein identified infections with 94% sensitivity and >99% specificity in individuals with sub-clinical (asymptomatic or pre-symptomatic) infections. |COVID-19 Testing[MESH] |COVID-19/*diagnosis/metabolism/*virology[MESH] |Carrier State/*diagnosis/*virology[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/methods[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH] |Saliva/*virology[MESH] |Sensitivity and Specificity[MESH]Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers(epmc).pdf DeepDyve Pubget Overpricing