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10.3389/fimmu.2021.635677

http://scihub22266oqcxt.onion/10.3389/fimmu.2021.635677
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suck abstract from ncbi


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pmid33777026      Front+Immunol 2021 ; 12 (ä): 635677
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  • Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein #MMPMID33777026
  • Li G; Wang A; Chen Y; Sun Y; Du Y; Wang X; Ding P; Jia R; Wang Y; Zhang G
  • Front Immunol 2021[]; 12 (ä): 635677 PMID33777026show ga
  • The outbreak and worldwide pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have a significant impact on global economy and human health. In order to reduce the disease spread, 16 monoclonal antibodies (McAbs) again SARS-CoV-2 were generated by immunized mice with the spike protein receptor binding domain (RBD), which was expressed in Chinese hamster ovary cell (CHO). A colloidal gold-based immunochromatographic strip was developed with two McAbs to detect SARS-CoV-2 spike protein, which can play a potential role in monitoring vaccine quality. The strip is highly specific, detecting only SARS-CoV-2 spike protein, and does not show any non-specific reactions with syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV) and other coronavirus and influenza viruses. The strip detected subunit vaccine in our laboratory with a detection limit of spike protein of 62.5 ng/mL. This strip provides an effective method in monitoring vaccine quality by detecting the antigen content of spike protein.
  • |*Gold Colloid[MESH]
  • |*Reagent Strips[MESH]
  • |Antibodies, Monoclonal/immunology[MESH]
  • |Antibody Specificity[MESH]
  • |Antigens, Viral/*analysis/immunology[MESH]
  • |COVID-19 Testing/*instrumentation[MESH]
  • |COVID-19/*diagnosis/immunology/virology[MESH]
  • |Humans[MESH]
  • |Immunoassay/*instrumentation[MESH]
  • |Limit of Detection[MESH]
  • |Predictive Value of Tests[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2/immunology/*metabolism[MESH]


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