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10.1016/j.jmoldx.2021.03.003 http://scihub22266oqcxt.onion/10.1016/j.jmoldx.2021.03.003 33775845!7997259!33775845     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Mol+Diagn 2021 ; 23 (6): 691-697 Nephropedia Template TP {{tp|p=33775845|t=2021. Prolonged Unfrozen Storage and Repeated Freeze-Thawing of SARS-CoV-2 Patient Samples Have Minor Effects on SARS-CoV-2 Detectability by RT-PCR |pdf=|usr=}}{{33775845}} gab.com Text Prolonged Unfrozen Storage and Repeated Freeze-Thawing of SARS-CoV-2 Patient Samples Have Minor Effects on SARS-CoV-2 Detectability by RT-PCR JO: J Mol Diagn http://www.kidney.de/pget.php?&tw=1&pmid=33775845 #FOAvip Reliable transportation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) patient samples from a swabbing station to a diagnostics facility is essential for accurate results. Therefore, cooling or freezing the samples is recommended in case of longer transportation times. In this study, SARS-CoV-2 detectability by RT-PCR was assessed after prolonged unfrozen storage or repetitive freeze-thawing of SARS-CoV-2 samples. SARS-CoV-2-positive patient swabs stored in viral transport medium were exposed to different temperatures (4 degrees C, 25 degrees C, and 35 degrees C) and to repetitive freeze-thawing, to assess the effect of storage conditions on RT-PCR detection. SARS-CoV-2 RNA was still reliably detected by RT-PCR after 21 days of storage in viral transport medium, even when the samples had been stored at 35 degrees C. The maximum observed change in cycle threshold value per day was 0.046 (+/-0.019) at 35 degrees C, and the maximum observed change in cycle threshold value per freeze-thaw cycle per day was 0.197 (+/-0.06). Compared with storage at 4 degrees C, viral RNA levels deviated little but significantly when stored at 25 degrees C or 35 degrees C, or after repeated freeze-thawing. The results of this study indicate that viral RNA levels are relatively stable at higher temperatures and repetitive freeze-thawing. Twit Text FOAVip Prolonged Unfrozen Storage and Repeated Freeze-Thawing of SARS-CoV-2 Patient Samples Have Minor Effects on SARS-CoV-2 Detectability by RT-PCR ????J Mol Diagn http://www.kidney.de/pget.php?&tw=1&pmid=33775845 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33775845 #FOAvip English Wikipedia <ref>{{Cite pmid|33775845}}</ref> Prolonged Unfrozen Storage and Repeated Freeze-Thawing of SARS-CoV-2 Patient Samples Have Minor Effects on SARS-CoV-2 Detectability by RT-PCR #MMPMID33775845 Dzung A; Cheng PF; Stoffel C; Tastanova A; Turko P; Levesque MP; Bosshard PP J Mol Diagn 2021[Jun]; 23 (6): 691-697 PMID33775845show ga Reliable transportation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) patient samples from a swabbing station to a diagnostics facility is essential for accurate results. Therefore, cooling or freezing the samples is recommended in case of longer transportation times. In this study, SARS-CoV-2 detectability by RT-PCR was assessed after prolonged unfrozen storage or repetitive freeze-thawing of SARS-CoV-2 samples. SARS-CoV-2-positive patient swabs stored in viral transport medium were exposed to different temperatures (4 degrees C, 25 degrees C, and 35 degrees C) and to repetitive freeze-thawing, to assess the effect of storage conditions on RT-PCR detection. SARS-CoV-2 RNA was still reliably detected by RT-PCR after 21 days of storage in viral transport medium, even when the samples had been stored at 35 degrees C. The maximum observed change in cycle threshold value per day was 0.046 (+/-0.019) at 35 degrees C, and the maximum observed change in cycle threshold value per freeze-thaw cycle per day was 0.197 (+/-0.06). Compared with storage at 4 degrees C, viral RNA levels deviated little but significantly when stored at 25 degrees C or 35 degrees C, or after repeated freeze-thawing. The results of this study indicate that viral RNA levels are relatively stable at higher temperatures and repetitive freeze-thawing. |COVID-19 Nucleic Acid Testing/instrumentation/methods/*standards[MESH] |COVID-19/*diagnosis/epidemiology[MESH] |Freezing[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |RNA Stability[MESH] |RNA, Viral/*genetics[MESH] |Real-Time Polymerase Chain Reaction/*standards[MESH] |SARS-CoV-2/*genetics[MESH] |Specimen Handling/*methods[MESH] |Switzerland/epidemiology[MESH] |Temperature[MESH]Prolonged Unfrozen Storage and Repeated Freeze-Thawing of SARS-CoV-2 P(epmc).pdf DeepDyve Pubget Overpricing