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10.1016/j.jviromet.2021.114143 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114143 33774075!7989071!33774075     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 294 (ä): 114143 Nephropedia Template TP {{tp|p=33774075|t=2021. A novel single nucleotide polymorphism assay for the detection of N501Y SARS-CoV-2 variants |pdf=|usr=}}{{33774075}} gab.com Text A novel single nucleotide polymorphism assay for the detection of N501Y SARS-CoV-2 variants JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33774075 #FOAvip The N501Y mutation in SARS-CoV-2 variants found in several strains from the UK, South Africa and Brazil has been linked to increased transmission. In order to discriminate N501Y variants quickly, a single nucleotide polymorphism (SNP) discrimination assay was designed and validated. It was then deployed prospectively in 757 nasopharyngeal swabs. Validation of the novel variant discrimination assay corroborated the results in all validation panel samples (n = 63) through sequencing. This novel variant discrimination assay was then deployed prospectively in 757 clinical nasopharyngeal swabs during the last week of January 2021. N501Y was found in 206 (27.4 %) of the samples: 94 (28.2 %) men and 112 (26.85 %) women (p = 0.73). The patients in whom it was identified had a mean age of 47.8 +/- 25.8 (0-96) years, similar to that of patients without this variant: 51.7 +/- 25.9 (0-104) years (p = 0.06). 501Y variant was confirmed in 34 samples by sequence method and 501 N wild type was confirmed in 67. This method is sensitive, specific, and simple to apply in any microbiology lab. Twit Text FOAVip A novel single nucleotide polymorphism assay for the detection of N501Y SARS-CoV-2 variants ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33774075 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33774075 #FOAvip English Wikipedia <ref>{{Cite pmid|33774075}}</ref> A novel single nucleotide polymorphism assay for the detection of N501Y SARS-CoV-2 variants #MMPMID33774075 Sandoval Torrientes M; Castello Abietar C; Boga Riveiro J; Alvarez-Arguelles ME; Rojo-Alba S; Abreu Salinas F; Costales Gonzalez I; Perez Martinez Z; Martin Rodriguez G; Gomez de Ona J; Coto Garcia E; Melon Garcia S J Virol Methods 2021[Aug]; 294 (ä): 114143 PMID33774075show ga The N501Y mutation in SARS-CoV-2 variants found in several strains from the UK, South Africa and Brazil has been linked to increased transmission. In order to discriminate N501Y variants quickly, a single nucleotide polymorphism (SNP) discrimination assay was designed and validated. It was then deployed prospectively in 757 nasopharyngeal swabs. Validation of the novel variant discrimination assay corroborated the results in all validation panel samples (n = 63) through sequencing. This novel variant discrimination assay was then deployed prospectively in 757 clinical nasopharyngeal swabs during the last week of January 2021. N501Y was found in 206 (27.4 %) of the samples: 94 (28.2 %) men and 112 (26.85 %) women (p = 0.73). The patients in whom it was identified had a mean age of 47.8 +/- 25.8 (0-96) years, similar to that of patients without this variant: 51.7 +/- 25.9 (0-104) years (p = 0.06). 501Y variant was confirmed in 34 samples by sequence method and 501 N wild type was confirmed in 67. This method is sensitive, specific, and simple to apply in any microbiology lab. |*Mutation[MESH] |*Polymorphism, Single Nucleotide[MESH] |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Child[MESH] |Child, Preschool[MESH] |Female[MESH] |Humans[MESH] |Infant[MESH] |Infant, Newborn[MESH] |Male[MESH] |Middle Aged[MESH] |Nasopharynx/virology[MESH] |Prospective Studies[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Sensitivity and Specificity[MESH] |Spike Glycoprotein, Coronavirus/genetics[MESH]A novel single nucleotide polymorphism assay for the detection of N501(epmc).pdf DeepDyve Pubget Overpricing