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Single virus inductively coupled plasma mass spectroscopy analysis: A comprehensive study #MMPMID33773712
Degueldre C
Talanta 2021[Jun]; 228 (?): 122211 PMID33773712show ga
The characterisation of individual nanoparticles by single particle ICP-MS (SP-ICP-MS) has paved the way for the analysis of smallest biological systems. This study suggests to adapting this method for single viruses (SV) identification and counting. With high resolution multi-channel sector field (MC SF) ICP-MS records in SV detection mode, the counting of master and key ions can allow analysis and identification of single viruses. The counting of 2-500 virial units can be performed in 20 s. Analyses are proposed to be carried out in Ar torch for master ions: (12)C(+), (13)C(+), (14)N(+), (15)N(+), and key ions (31)P(+), (32)S(+), (33)S(+) and (34)S(+). All interferences are discussed in detail. The use of high resolution SF ICP-MS is recommended while options with anaerobic/aerobic atmospheres are explored to upgrade the analysis when using quadrupole ICP-MS. Application for two virus types (SARS-COV2 and bacteriophage T5) is investigated using time scan and fixed mass analysis for the selected virus ions allowing characterisation of the species using the N/C, P/C and S/C molar ratio's and quantification of their number concentration.
Talanta 2021 ; 228 (?): 122211
