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10.1002/eji.202049101

http://scihub22266oqcxt.onion/10.1002/eji.202049101
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33772767!8250065!33772767
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suck abstract from ncbi


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pmid33772767      Eur+J+Immunol 2021 ; 51 (7): 1839-1849
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  • Peptide microarray-based analysis of antibody responses to SARS-CoV-2 identifies unique epitopes with potential for diagnostic test development #MMPMID33772767
  • Holenya P; Lange PJ; Reimer U; Woltersdorf W; Panterodt T; Glas M; Wasner M; Eckey M; Drosch M; Hollidt JM; Naumann M; Kern F; Wenschuh H; Lange R; Schnatbaum K; Bier FF
  • Eur J Immunol 2021[Jul]; 51 (7): 1839-1849 PMID33772767show ga
  • Humoral immunity to the Severe Adult Respiratory Syndrome (SARS) Coronavirus (CoV)-2 is not fully understood yet but is a crucial factor of immune protection. The possibility of antibody cross-reactivity between SARS-CoV-2 and other human coronaviruses (HCoVs) would have important implications for immune protection but also for the development of specific diagnostic ELISA tests. Using peptide microarrays, n = 24 patient samples and n = 12 control samples were screened for antibodies against the entire SARS-CoV-2 proteome as well as the Spike (S), Nucleocapsid (N), VME1 (V), R1ab, and Protein 3a (AP3A) of the HCoV strains SARS, MERS, OC43, and 229E. While widespread cross-reactivity was revealed across several immunodominant regions of S and N, IgG binding to several SARS-CoV-2-derived peptides provided statistically significant discrimination between COVID-19 patients and controls. Selected target peptides may serve as capture antigens for future, highly COVID-19-specific diagnostic antibody tests.
  • |Adult[MESH]
  • |Aged[MESH]
  • |Amino Acid Sequence/genetics[MESH]
  • |Antibodies, Viral/*blood/immunology[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Coronavirus 229E, Human/immunology[MESH]
  • |Coronavirus Nucleocapsid Proteins/immunology[MESH]
  • |Coronavirus OC43, Human/immunology[MESH]
  • |Cross Reactions/immunology[MESH]
  • |Diagnostic Tests, Routine[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoglobulin G/blood/immunology[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Middle East Respiratory Syndrome Coronavirus/immunology[MESH]
  • |Phosphoproteins/immunology[MESH]
  • |Protein Array Analysis/*methods[MESH]
  • |Proteome/immunology[MESH]
  • |SARS-CoV-2/*immunology[MESH]
  • |Severe acute respiratory syndrome-related coronavirus/immunology[MESH]
  • |Spike Glycoprotein, Coronavirus/immunology[MESH]
  • |Viral Proteins/*immunology[MESH]


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