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10.1038/s41467-021-22072-9 http://scihub22266oqcxt.onion/10.1038/s41467-021-22072-9 33771993!7997897!33771993     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33771993&cmd=llinks): Failed to open stream: HTTP request failed! 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N-protein presents early in blood, dried blood and saliva during asymptomatic and symptomatic SARS-CoV-2 infection |pdf=|usr=}}{{33771993}} gab.com Text N-protein presents early in blood, dried blood and saliva during asymptomatic and symptomatic SARS-CoV-2 infection JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33771993 #FOAvip The COVID-19 pandemic continues to have an unprecedented impact on societies and economies worldwide. There remains an ongoing need for high-performance SARS-CoV-2 tests which may be broadly deployed for infection monitoring. Here we report a highly sensitive single molecule array (Simoa) immunoassay in development for detection of SARS-CoV-2 nucleocapsid protein (N-protein) in venous and capillary blood and saliva. In all matrices in the studies conducted to date we observe >98% negative percent agreement and >90% positive percent agreement with molecular testing for days 1-7 in symptomatic, asymptomatic, and pre-symptomatic PCR+ individuals. N-protein load decreases as anti-SARS-CoV-2 spike-IgG increases, and N-protein levels correlate with RT-PCR Ct-values in saliva, and between matched saliva and capillary blood samples. This Simoa SARS-CoV-2 N-protein assay effectively detects SARS-CoV-2 infection via measurement of antigen levels in blood or saliva, using non-invasive, swab-independent collection methods, offering potential for at home and point of care sample collection. Twit Text FOAVip N-protein presents early in blood, dried blood and saliva during asymptomatic and symptomatic SARS-CoV-2 infection ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33771993 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33771993 #FOAvip English Wikipedia <ref>{{Cite pmid|33771993}}</ref> N-protein presents early in blood, dried blood and saliva during asymptomatic and symptomatic SARS-CoV-2 infection #MMPMID33771993 Shan D; Johnson JM; Fernandes SC; Suib H; Hwang S; Wuelfing D; Mendes M; Holdridge M; Burke EM; Beauregard K; Zhang Y; Cleary M; Xu S; Yao X; Patel PP; Plavina T; Wilson DH; Chang L; Kaiser KM; Nattermann J; Schmidt SV; Latz E; Hrusovsky K; Mattoon D; Ball AJ Nat Commun 2021[Mar]; 12 (1): 1931 PMID33771993show ga The COVID-19 pandemic continues to have an unprecedented impact on societies and economies worldwide. There remains an ongoing need for high-performance SARS-CoV-2 tests which may be broadly deployed for infection monitoring. Here we report a highly sensitive single molecule array (Simoa) immunoassay in development for detection of SARS-CoV-2 nucleocapsid protein (N-protein) in venous and capillary blood and saliva. In all matrices in the studies conducted to date we observe >98% negative percent agreement and >90% positive percent agreement with molecular testing for days 1-7 in symptomatic, asymptomatic, and pre-symptomatic PCR+ individuals. N-protein load decreases as anti-SARS-CoV-2 spike-IgG increases, and N-protein levels correlate with RT-PCR Ct-values in saliva, and between matched saliva and capillary blood samples. This Simoa SARS-CoV-2 N-protein assay effectively detects SARS-CoV-2 infection via measurement of antigen levels in blood or saliva, using non-invasive, swab-independent collection methods, offering potential for at home and point of care sample collection. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/epidemiology/virology[MESH] |Coronavirus Nucleocapsid Proteins/*blood/genetics[MESH] |Epidemics[MESH] |Home Care Services[MESH] |Humans[MESH] |Point-of-Care Systems[MESH] |ROC Curve[MESH] |SARS-CoV-2/genetics/*metabolism/physiology[MESH] |Saliva/*virology[MESH]N-protein presents early in blood, dried blood and saliva during asymp(epmc).pdf DeepDyve Pubget Overpricing