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10.1016/j.jcv.2021.104797 http://scihub22266oqcxt.onion/10.1016/j.jcv.2021.104797 33770657!7968170!33770657     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33770657&cmd=llinks): Failed to open stream: HTTP request failed! 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An evaluation of 4 commercial assays for the detection of SARS-CoV-2 antibodies in a predominantly mildly symptomatic low prevalence Australian population |pdf=|usr=}}{{33770657}} gab.com Text An evaluation of 4 commercial assays for the detection of SARS-CoV-2 antibodies in a predominantly mildly symptomatic low prevalence Australian population JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=33770657 #FOAvip A total of 1080 individual patient samples (158 positive serology samples from confirmed, predominantly mildly symptomatic COVID-19 patients and 922 serology negative including 496 collected pre-COVID) from four states in Australia were analysed on four commercial SARS-CoV-2 serological assays targeting antibodies to different antigens (Roche Elecsys and Abbott Architect: nucleocapsid; Diasorin Liaison and Euroimmun: spike). A subset was compared to immunofluorescent antibody (IFA) and micro-neutralisation. Sensitivity and specificity of the Roche (n = 1033), Abbott (n = 806), Diasorin (n = 1034) and Euroimmun (n = 175) were 93.7 %/99.5 %, 90.2 %/99.4 %, 88.6 %/98.6 % and 91.3 %/98.8 %, respectively. ROC analysis with specificity held at 99 % increased the sensitivity for the Roche and Abbott assays from 93.7% to 98.7% (cut-off 0.21) and 90.2 % to 94.0 % (cut-off 0.91), respectively. Overall seropositivity of samples increased from a maximum of 23 % for samples 0-7 days-post-onset of symptoms (dpos), to 61 % from samples 8-14dpos and 93 % from those >14dpos. IFA and microneutralisation values correlated best with assays targeting antibodies to spike protein with values >80 AU/mL on the Diasorin assay associated with neutralising antibody. Detectable antibody was present in 22/23 (96 %), 20/23 (87 %), 15/23 (65 %) and 9/22 (41 %) patients with samples >180dpos on the Roche, Diasorin, Abbott and microneutralisation assays respectively. Given the low prevalence in this community, two-step algorithms on initial positive results saw an increase in the positive predictive value (PPV) of positive samples (39 %-65 % to >/=98 %) for all combinations. Similarly accuracy increased from a range of 98.5 %-99.4 % to >/=99.8 % assuming a 1 % seroprevalence. Negative predictive value (NPV) was high (>/=99.8 %) regardless of which assay was used initially. Twit Text FOAVip An evaluation of 4 commercial assays for the detection of SARS-CoV-2 antibodies in a predominantly mildly symptomatic low prevalence Australian population ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=33770657 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33770657 #FOAvip English Wikipedia <ref>{{Cite pmid|33770657}}</ref> An evaluation of 4 commercial assays for the detection of SARS-CoV-2 antibodies in a predominantly mildly symptomatic low prevalence Australian population #MMPMID33770657 Wehrhahn MC; Brown SJ; Newcombe JP; Chong S; Evans J; Figtree M; Hainke L; Hueston L; Khan S; Marland E; O'Sullivan MVN; Powell H; Roy J; Waring L; Yu M; Robson J J Clin Virol 2021[May]; 138 (ä): 104797 PMID33770657show ga A total of 1080 individual patient samples (158 positive serology samples from confirmed, predominantly mildly symptomatic COVID-19 patients and 922 serology negative including 496 collected pre-COVID) from four states in Australia were analysed on four commercial SARS-CoV-2 serological assays targeting antibodies to different antigens (Roche Elecsys and Abbott Architect: nucleocapsid; Diasorin Liaison and Euroimmun: spike). A subset was compared to immunofluorescent antibody (IFA) and micro-neutralisation. Sensitivity and specificity of the Roche (n = 1033), Abbott (n = 806), Diasorin (n = 1034) and Euroimmun (n = 175) were 93.7 %/99.5 %, 90.2 %/99.4 %, 88.6 %/98.6 % and 91.3 %/98.8 %, respectively. ROC analysis with specificity held at 99 % increased the sensitivity for the Roche and Abbott assays from 93.7% to 98.7% (cut-off 0.21) and 90.2 % to 94.0 % (cut-off 0.91), respectively. Overall seropositivity of samples increased from a maximum of 23 % for samples 0-7 days-post-onset of symptoms (dpos), to 61 % from samples 8-14dpos and 93 % from those >14dpos. IFA and microneutralisation values correlated best with assays targeting antibodies to spike protein with values >80 AU/mL on the Diasorin assay associated with neutralising antibody. Detectable antibody was present in 22/23 (96 %), 20/23 (87 %), 15/23 (65 %) and 9/22 (41 %) patients with samples >180dpos on the Roche, Diasorin, Abbott and microneutralisation assays respectively. Given the low prevalence in this community, two-step algorithms on initial positive results saw an increase in the positive predictive value (PPV) of positive samples (39 %-65 % to >/=98 %) for all combinations. Similarly accuracy increased from a range of 98.5 %-99.4 % to >/=99.8 % assuming a 1 % seroprevalence. Negative predictive value (NPV) was high (>/=99.8 %) regardless of which assay was used initially. |*Reagent Kits, Diagnostic[MESH] |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Antibodies, Viral/*blood[MESH] |Australia/epidemiology[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/*diagnosis/epidemiology[MESH] |Child[MESH] |Coronavirus Nucleocapsid Proteins/immunology[MESH] |Female[MESH] |Humans[MESH] |Immunoglobulin Isotypes/blood[MESH] |Male[MESH] |Middle Aged[MESH] |Phosphoproteins/immunology[MESH] |Prevalence[MESH] |Sensitivity and Specificity[MESH] |Seroepidemiologic Studies[MESH] |Spike Glycoprotein, Coronavirus/immunology[MESH]An evaluation of 4 commercial assays for the detection of SARS-CoV-2 a(epmc).pdf DeepDyve Pubget Overpricing