Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1007/s00216-021-03298-4 http://scihub22266oqcxt.onion/10.1007/s00216-021-03298-4 33768365!7993413!33768365     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Bioanal+Chem 2021 ; 413 (13): 3501-3510 Nephropedia Template TP {{tp|p=33768365|t=2021. Spike vs nucleocapsid SARS-CoV-2 antigen detection: application in nasopharyngeal swab specimens |pdf=|usr=}}{{33768365}} gab.com Text Spike vs nucleocapsid SARS-CoV-2 antigen detection: application in nasopharyngeal swab specimens JO: Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=33768365 #FOAvip Public health experts emphasize the need for quick, point-of-care SARS-CoV-2 detection as an effective strategy for controlling virus spread. To this end, many "antigen" detection devices were developed and commercialized. These devices are mostly based on detecting SARS-CoV-2's nucleocapsid protein. Recently, alerts issued by both the FDA and the CDC raised concerns regarding the devices' tendency to exhibit false positive results. In this work, we developed a novel alternative spike-based antigen assay, comprising four high-affinity, specific monoclonal antibodies, directed against different epitopes on the spike's S1 subunit. The assay's performance was evaluated for COVID-19 detection from nasopharyngeal swabs, compared to an in-house nucleocapsid-based assay, composed of novel antibodies directed against the nucleocapsid. Detection of COVID-19 was carried out in a cohort of 284 qRT-PCR positive and negative nasopharyngeal swab samples. The time resolved fluorescence (TRF) ELISA spike assay displayed very high specificity (99%) accompanied with a somewhat lower sensitivity (66% for Ct < 25), compared to the nucleocapsid ELISA assay which was more sensitive (85% for Ct < 25) while less specific (87% specificity). Despite being outperformed by qRT-PCR, we suggest that there is room for such tests in the clinical setting, as cheap and rapid pre-screening tools. Our results further suggest that when applying antigen detection, one must consider its intended application (sensitivity vs specificity), taking into consideration that the nucleocapsid might not be the optimal target. In this regard, we propose that a combination of both antigens might contribute to the validity of the results. Schematic representation of sample collection and analysis. The figure was created using BioRender.com. Twit Text FOAVip Spike vs nucleocapsid SARS-CoV-2 antigen detection: application in nasopharyngeal swab specimens ????Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=33768365 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33768365 #FOAvip English Wikipedia <ref>{{Cite pmid|33768365}}</ref> Spike vs nucleocapsid SARS-CoV-2 antigen detection: application in nasopharyngeal swab specimens #MMPMID33768365 Barlev-Gross M; Weiss S; Ben-Shmuel A; Sittner A; Eden K; Mazuz N; Glinert I; Bar-David E; Puni R; Amit S; Kriger O; Schuster O; Alcalay R; Makdasi E; Epstein E; Noy-Porat T; Rosenfeld R; Achdout H; Mazor O; Israely T; Levy H; Mechaly A Anal Bioanal Chem 2021[May]; 413 (13): 3501-3510 PMID33768365show ga Public health experts emphasize the need for quick, point-of-care SARS-CoV-2 detection as an effective strategy for controlling virus spread. To this end, many "antigen" detection devices were developed and commercialized. These devices are mostly based on detecting SARS-CoV-2's nucleocapsid protein. Recently, alerts issued by both the FDA and the CDC raised concerns regarding the devices' tendency to exhibit false positive results. In this work, we developed a novel alternative spike-based antigen assay, comprising four high-affinity, specific monoclonal antibodies, directed against different epitopes on the spike's S1 subunit. The assay's performance was evaluated for COVID-19 detection from nasopharyngeal swabs, compared to an in-house nucleocapsid-based assay, composed of novel antibodies directed against the nucleocapsid. Detection of COVID-19 was carried out in a cohort of 284 qRT-PCR positive and negative nasopharyngeal swab samples. The time resolved fluorescence (TRF) ELISA spike assay displayed very high specificity (99%) accompanied with a somewhat lower sensitivity (66% for Ct < 25), compared to the nucleocapsid ELISA assay which was more sensitive (85% for Ct < 25) while less specific (87% specificity). Despite being outperformed by qRT-PCR, we suggest that there is room for such tests in the clinical setting, as cheap and rapid pre-screening tools. Our results further suggest that when applying antigen detection, one must consider its intended application (sensitivity vs specificity), taking into consideration that the nucleocapsid might not be the optimal target. In this regard, we propose that a combination of both antigens might contribute to the validity of the results. Schematic representation of sample collection and analysis. The figure was created using BioRender.com. |COVID-19 Serological Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Coronavirus Nucleocapsid Proteins/*analysis[MESH] |Enzyme-Linked Immunosorbent Assay/methods[MESH] |Humans[MESH] |Phosphoproteins/analysis[MESH] |SARS-CoV-2/*isolation & purification[MESH] |Sensitivity and Specificity[MESH] |Specimen Handling[MESH]Spike vs nucleocapsid SARS-CoV-2 antigen detection: application in nas(epmc).pdf DeepDyve Pubget Overpricing