Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1073/pnas.2025866118 http://scihub22266oqcxt.onion/10.1073/pnas.2025866118 33766889!8053989!33766889     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2021 ; 118 (15): ä Nephropedia Template TP {{tp|p=33766889|t=2021. Generation of SARS-CoV-2 reporter replicon for high-throughput antiviral screening and testing |pdf=|usr=}}{{33766889}} gab.com Text Generation of SARS-CoV-2 reporter replicon for high-throughput antiviral screening and testing JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=33766889 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research and antiviral discovery are hampered by the lack of a cell-based virus replication system that can be readily adopted without biosafety level 3 (BSL-3) restrictions. Here, the construction of a noninfectious SARS-CoV-2 reporter replicon and its application in deciphering viral replication mechanisms and evaluating SARS-CoV-2 inhibitors are presented. The replicon genome is replication competent but does not produce progeny virions. Its replication can be inhibited by RdRp mutations or by known SARS-CoV-2 antiviral compounds. Using this system, a high-throughput antiviral assay has also been developed. Significant differences in potencies of several SARS-CoV-2 inhibitors in different cell lines were observed, which highlight the challenges of discovering antivirals capable of inhibiting viral replication in vivo and the importance of testing compounds in multiple cell culture models. The generation of a SARS-CoV-2 replicon provides a powerful platform to expand the global research effort to combat COVID-19. Twit Text FOAVip Generation of SARS-CoV-2 reporter replicon for high-throughput antiviral screening and testing ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=33766889 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33766889 #FOAvip English Wikipedia <ref>{{Cite pmid|33766889}}</ref> Generation of SARS-CoV-2 reporter replicon for high-throughput antiviral screening and testing #MMPMID33766889 He X; Quan S; Xu M; Rodriguez S; Goh SL; Wei J; Fridman A; Koeplinger KA; Carroll SS; Grobler JA; Espeseth AS; Olsen DB; Hazuda DJ; Wang D Proc Natl Acad Sci U S A 2021[Apr]; 118 (15): ä PMID33766889show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research and antiviral discovery are hampered by the lack of a cell-based virus replication system that can be readily adopted without biosafety level 3 (BSL-3) restrictions. Here, the construction of a noninfectious SARS-CoV-2 reporter replicon and its application in deciphering viral replication mechanisms and evaluating SARS-CoV-2 inhibitors are presented. The replicon genome is replication competent but does not produce progeny virions. Its replication can be inhibited by RdRp mutations or by known SARS-CoV-2 antiviral compounds. Using this system, a high-throughput antiviral assay has also been developed. Significant differences in potencies of several SARS-CoV-2 inhibitors in different cell lines were observed, which highlight the challenges of discovering antivirals capable of inhibiting viral replication in vivo and the importance of testing compounds in multiple cell culture models. The generation of a SARS-CoV-2 replicon provides a powerful platform to expand the global research effort to combat COVID-19. |A549 Cells[MESH] |Animals[MESH] |Antiviral Agents/*pharmacology[MESH] |COVID-19/*virology[MESH] |Chlorocebus aethiops[MESH] |Coronavirus RNA-Dependent RNA Polymerase/genetics[MESH] |HEK293 Cells[MESH] |High-Throughput Screening Assays/*methods[MESH] |Humans[MESH] |Replicon/*drug effects/genetics[MESH] |SARS-CoV-2/*drug effects/genetics[MESH] |Vero Cells[MESH]Generation of SARS-CoV-2 reporter replicon for high-throughput antivir(epmc).pdf DeepDyve Pubget Overpricing