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10.1016/j.diagmicrobio.2021.115366

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suck abstract from ncbi


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pmid33756311      Diagn+Microbiol+Infect+Dis 2021 ; 100 (3): 115366
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  • Caution in interpretation of SARS-CoV-2 quantification based on RT-PCR cycle threshold value #MMPMID33756311
  • Schnuriger A; Perrier M; Marinho V; Michel Y; Saloum K; Boukli N; Lambert-Niclot S; Amiel C; Fofana DB; Gozlan J; Morand-Joubert L
  • Diagn Microbiol Infect Dis 2021[Jul]; 100 (3): 115366 PMID33756311show ga
  • RT-PCR is the reference method for diagnosis of a Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection. During the setting up of 6 SARS-CoV-2 RT-PCR assays in our laboratory, comparative evaluations were systematically undertaken and allowed to evidence major discrepancies on cycle threshold RT-PCR results between techniques. These tendencies were confirmed in routine application when analyzing sequential samples from the same patients. Our aim was to examine the impact of the technique among factors influencing RT-PCR result, a far surrogate of 'viral load' in the heterogeneous environment of respiratory specimens.
  • |COVID-19 Nucleic Acid Testing/methods/*statistics & numerical data[MESH]
  • |COVID-19/diagnosis/*virology[MESH]
  • |Datasets as Topic[MESH]
  • |Diagnostic Tests, Routine[MESH]
  • |Genome, Viral[MESH]
  • |Humans[MESH]
  • |Nasopharynx/virology[MESH]
  • |RNA, Viral/genetics[MESH]
  • |SARS-CoV-2/genetics/*isolation & purification[MESH]


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