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10.1371/journal.pone.0248371 http://scihub22266oqcxt.onion/10.1371/journal.pone.0248371 33755704!7987156!33755704     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2021 ; 16 (3): e0248371 Nephropedia Template TP {{tp|p=33755704|t=2021. Whole-genome sequencing of SARS-CoV-2 reveals the detection of G614 variant in Pakistan |pdf=|usr=}}{{33755704}} gab.com Text Whole-genome sequencing of SARS-CoV-2 reveals the detection of G614 variant in Pakistan JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33755704 #FOAvip Since its emergence in China, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide including Pakistan. During the pandemic, whole genome sequencing has played an important role in understanding the evolution and genomic diversity of SARS-CoV-2. Although an unprecedented number of SARS-CoV-2 full genomes have been submitted in GISAID and NCBI, data from Pakistan is scarce. We report the sequencing, genomic characterization, and phylogenetic analysis of five SARS-CoV-2 strains isolated from patients in Pakistan. The oropharyngeal swabs of patients that were confirmed positive for SARS-CoV-2 through real-time RT-PCR at National Institute of Health, Pakistan, were selected for whole-genome sequencing. Sequencing was performed using NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEW ENGLAND BioLabs Inc., MA, US) and Illumina iSeq 100 instrument (Illumina, San Diego, US). Based on whole-genome analysis, three Pakistani SARS-CoV-2 strains clustered into the 20A (GH) clade along with the strains from Oman, Slovakia, United States, and Pakistani strain EPI_ISL_513925. The two 19B (S)-clade strains were closely related to viruses from India and Oman. Overall, twenty-nine amino acid mutations were detected in the current study genome sequences, including fifteen missense and four novel mutations. Notably, we have found a D614G (aspartic acid to glycine) mutation in spike protein of the sequences from the GH clade. The G614 variant carrying the characteristic D614G mutation has been shown to be more infectious that lead to its rapid spread worldwide. This report highlights the detection of GH and S clade strains and G614 variant from Pakistan warranting large-scale whole-genome sequencing of strains prevalent in different regions to understand virus evolution and to explore their genetic diversity. Twit Text FOAVip Whole-genome sequencing of SARS-CoV-2 reveals the detection of G614 variant in Pakistan ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33755704 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33755704 #FOAvip English Wikipedia <ref>{{Cite pmid|33755704}}</ref> Whole-genome sequencing of SARS-CoV-2 reveals the detection of G614 variant in Pakistan #MMPMID33755704 Umair M; Ikram A; Salman M; Khurshid A; Alam M; Badar N; Suleman R; Tahir F; Sharif S; Montgomery J; Whitmer S; Klena J PLoS One 2021[]; 16 (3): e0248371 PMID33755704show ga Since its emergence in China, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide including Pakistan. During the pandemic, whole genome sequencing has played an important role in understanding the evolution and genomic diversity of SARS-CoV-2. Although an unprecedented number of SARS-CoV-2 full genomes have been submitted in GISAID and NCBI, data from Pakistan is scarce. We report the sequencing, genomic characterization, and phylogenetic analysis of five SARS-CoV-2 strains isolated from patients in Pakistan. The oropharyngeal swabs of patients that were confirmed positive for SARS-CoV-2 through real-time RT-PCR at National Institute of Health, Pakistan, were selected for whole-genome sequencing. Sequencing was performed using NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEW ENGLAND BioLabs Inc., MA, US) and Illumina iSeq 100 instrument (Illumina, San Diego, US). Based on whole-genome analysis, three Pakistani SARS-CoV-2 strains clustered into the 20A (GH) clade along with the strains from Oman, Slovakia, United States, and Pakistani strain EPI_ISL_513925. The two 19B (S)-clade strains were closely related to viruses from India and Oman. Overall, twenty-nine amino acid mutations were detected in the current study genome sequences, including fifteen missense and four novel mutations. Notably, we have found a D614G (aspartic acid to glycine) mutation in spike protein of the sequences from the GH clade. The G614 variant carrying the characteristic D614G mutation has been shown to be more infectious that lead to its rapid spread worldwide. This report highlights the detection of GH and S clade strains and G614 variant from Pakistan warranting large-scale whole-genome sequencing of strains prevalent in different regions to understand virus evolution and to explore their genetic diversity. |*Genetic Variation[MESH] |Aged, 80 and over[MESH] |COVID-19/pathology/virology[MESH] |Female[MESH] |Gene Deletion[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Mutation, Missense[MESH] |Oropharynx/virology[MESH] |Pakistan[MESH] |Phylogeny[MESH] |RNA, Viral/chemistry/isolation & purification/metabolism[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2/classification/*genetics/isolation & purification[MESH] |Spike Glycoprotein, Coronavirus/*genetics/metabolism[MESH] |Whole Genome Sequencing[MESH]Whole-genome sequencing of SARS-CoV-2 reveals the detection of G614 va(epmc).pdf DeepDyve Pubget Overpricing