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10.1038/s41467-021-22102-6 http://scihub22266oqcxt.onion/10.1038/s41467-021-22102-6 33753733!7985487!33753733     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 1806 Nephropedia Template TP {{tp|p=33753733|t=2021. A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies |pdf=|usr=}}{{33753733}} gab.com Text A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33753733 #FOAvip Better diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc component. The assay is performed directly in the liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that SATiN has a similar sensitivity to ELISA, and its readouts are consistent with various neutralizing antibody assays. This proof-of-principle study suggests potential applications in diagnostics, as well as disease and vaccination management. Twit Text FOAVip A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33753733 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33753733 #FOAvip English Wikipedia <ref>{{Cite pmid|33753733}}</ref> A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies #MMPMID33753733 Yao Z; Drecun L; Aboualizadeh F; Kim SJ; Li Z; Wood H; Valcourt EJ; Manguiat K; Plenderleith S; Yip L; Li X; Zhong Z; Yue FY; Closas T; Snider J; Tomic J; Drews SJ; Drebot MA; McGeer A; Ostrowski M; Mubareka S; Rini JM; Owen S; Stagljar I Nat Commun 2021[Mar]; 12 (1): 1806 PMID33753733show ga Better diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc component. The assay is performed directly in the liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that SATiN has a similar sensitivity to ELISA, and its readouts are consistent with various neutralizing antibody assays. This proof-of-principle study suggests potential applications in diagnostics, as well as disease and vaccination management. |Antibodies, Neutralizing/blood[MESH] |Antibodies, Viral/*blood/immunology[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/blood/*diagnosis/virology[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Immunoglobulin G/blood[MESH] |Immunoglobulin M/blood[MESH] |Luciferases/*metabolism[MESH] |SARS-CoV-2/*immunology/*isolation & purification[MESH]A homogeneous split-luciferase assay for rapid and sensitive detection(epmc).pdf DeepDyve Pubget Overpricing