Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/anie.202100352 http://scihub22266oqcxt.onion/10.1002/anie.202100352 33751748!8250829!33751748     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Angew+Chem+Int+Ed+Engl 2021 ; 60 (24): 13280-13286 Nephropedia Template TP {{tp|p=33751748|t=2021. Chemo-Enzymatic Modification of the 5 Cap Maintains Translation and Increases Immunogenic Properties of mRNA |pdf=|usr=}}{{33751748}} gab.com Text Chemo-Enzymatic Modification of the 5 Cap Maintains Translation and Increases Immunogenic Properties of mRNA JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=33751748 #FOAvip Eukaryotic mRNAs are emerging modalities for protein replacement therapy and vaccination. Their 5' cap is important for mRNA translation and immune response and can be naturally methylated at different positions by S-adenosyl-l-methionine (AdoMet)-dependent methyltransferases (MTases). We report on the cosubstrate scope of the MTase CAPAM responsible for methylation at the N(6) -position of adenosine start nucleotides using synthetic AdoMet analogs. The chemo-enzymatic propargylation enabled production of site-specifically modified reporter-mRNAs. These cap-propargylated mRNAs were efficiently translated and showed approximately 3-fold increased immune response in human cells. The same effects were observed when the receptor binding domain (RBD) of SARS-CoV-2-a currently tested epitope for mRNA vaccination-was used. Site-specific chemo-enzymatic modification of eukaryotic mRNA may thus be a suitable strategy to modulate translation and immune response of mRNAs for future therapeutic applications. Twit Text FOAVip Chemo-Enzymatic Modification of the 5 Cap Maintains Translation and Increases Immunogenic Properties of mRNA ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=33751748 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33751748 #FOAvip English Wikipedia <ref>{{Cite pmid|33751748}}</ref> Chemo-Enzymatic Modification of the 5 Cap Maintains Translation and Increases Immunogenic Properties of mRNA #MMPMID33751748 van Dulmen M; Muthmann N; Rentmeister A Angew Chem Int Ed Engl 2021[Jun]; 60 (24): 13280-13286 PMID33751748show ga Eukaryotic mRNAs are emerging modalities for protein replacement therapy and vaccination. Their 5' cap is important for mRNA translation and immune response and can be naturally methylated at different positions by S-adenosyl-l-methionine (AdoMet)-dependent methyltransferases (MTases). We report on the cosubstrate scope of the MTase CAPAM responsible for methylation at the N(6) -position of adenosine start nucleotides using synthetic AdoMet analogs. The chemo-enzymatic propargylation enabled production of site-specifically modified reporter-mRNAs. These cap-propargylated mRNAs were efficiently translated and showed approximately 3-fold increased immune response in human cells. The same effects were observed when the receptor binding domain (RBD) of SARS-CoV-2-a currently tested epitope for mRNA vaccination-was used. Site-specific chemo-enzymatic modification of eukaryotic mRNA may thus be a suitable strategy to modulate translation and immune response of mRNAs for future therapeutic applications. |COVID-19/pathology/virology[MESH] |Chromatography, High Pressure Liquid[MESH] |Genes, Reporter[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Mass Spectrometry[MESH] |Methylation[MESH] |Methyltransferases/metabolism[MESH] |Protein Biosynthesis[MESH] |Protein Domains/genetics/immunology[MESH] |RNA Caps/analysis/*immunology/metabolism[MESH] |RNA, Messenger/genetics/*immunology/metabolism[MESH] |S-Adenosylmethionine/chemistry/immunology/metabolism[MESH] |SARS-CoV-2/genetics/isolation & purification[MESH]Chemo-Enzymatic Modification of the 5 Cap Maintains Translation and I(epmc).pdf DeepDyve Pubget Overpricing