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10.1002/jmv.26926

http://scihub22266oqcxt.onion/10.1002/jmv.26926
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33751621!8207115!33751621
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suck abstract from ncbi


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pmid33751621      J+Med+Virol 2021 ; 93 (7): 4219-4241
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  • The next-generation coronavirus diagnostic techniques with particular emphasis on the SARS-CoV-2 #MMPMID33751621
  • Hemida MG
  • J Med Virol 2021[Jul]; 93 (7): 4219-4241 PMID33751621show ga
  • The potential zoonotic coronaviruses (SARS-CoV, MERS-CoV, and SARS-CoV-2) are of global health concerns. Early diagnosis is the milestone in their mitigation, control, and eradication. Many diagnostic techniques are showing great success and have many advantages, such as the rapid turnover of the results, high accuracy, and high specificity and sensitivity. However, some of these techniques have several pitfalls if samples were not collected, processed, and transported in the standard ways and if these techniques were not practiced with extreme caution and precision. This may lead to false-negative/positive results. This may affect the downstream management of the affected cases. These techniques require regular fine-tuning, upgrading, and optimization. The continuous evolution of new strains and viruses belong to the coronaviruses is hampering the success of many classical techniques. There are urgent needs for next generations of coronaviruses diagnostic assays that overcome these pitfalls. This new generation of diagnostic tests should be able to do simultaneous, multiplex, and high-throughput detection of various coronavirus in one reaction. Furthermore, the development of novel assays and techniques that enable the in situ detection of the virus on the environmental samples, especially air, water, and surfaces, should be given considerable attention in the future. These approaches will have a substantial positive impact on the mitigation and eradication of coronaviruses, including the current SARS-CoV-2 pandemic.
  • |COVID-19/*diagnosis[MESH]
  • |Enzyme-Linked Immunosorbent Assay/methods[MESH]
  • |Fluorescent Antibody Technique/methods[MESH]
  • |Genome, Viral/genetics[MESH]
  • |High-Throughput Screening Assays/*methods[MESH]
  • |Humans[MESH]
  • |Middle East Respiratory Syndrome Coronavirus/genetics[MESH]
  • |Molecular Diagnostic Techniques/methods[MESH]
  • |Nucleic Acid Amplification Techniques/methods[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction/methods[MESH]
  • |SARS-CoV-2/genetics/isolation & purification[MESH]
  • |Severe Acute Respiratory Syndrome/*diagnosis[MESH]
  • |Severe acute respiratory syndrome-related coronavirus/genetics[MESH]
  • |Spike Glycoprotein, Coronavirus/genetics[MESH]


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  • suck abstract from ncbi

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