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Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Wellcome+Open+Res 2020 ; 5 (ä): 139 Nephropedia Template TP
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Antibody testing for COVID-19: A report from the National COVID Scientific Advisory Panel #MMPMID33748431
Adams ER; Ainsworth M; Anand R; Andersson MI; Auckland K; Baillie JK; Barnes E; Beer S; Bell JI; Berry T; Bibi S; Carroll M; Chinnakannan SK; Clutterbuck E; Cornall RJ; Crook DW; de Silva T; Dejnirattisai W; Dingle KE; Dold C; Espinosa A; Eyre DW; Farmer H; Fernandez Mendoza M; Georgiou D; Hoosdally SJ; Hunter A; Jefferey K; Kelly DF; Klenerman P; Knight J; Knowles C; Kwok AJ; Leuschner U; Levin R; Liu C; Lopez-Camacho C; Martinez J; Matthews PC; McGivern H; Mentzer AJ; Milton J; Mongkolsapaya J; Moore SC; Oliveira MS; Pereira F; Perez E; Peto T; Ploeg RJ; Pollard A; Prince T; Roberts DJ; Rudkin JK; Sanchez V; Screaton GR; Semple MG; Slon-Campos J; Skelly DT; Smith EN; Sobrinodiaz A; Staves J; Stuart DI; Supasa P; Surik T; Thraves H; Tsang P; Turtle L; Walker AS; Wang B; Washington C; Watkins N; Whitehouse J
Wellcome Open Res 2020[]; 5 (ä): 139 PMID33748431show ga
Background: The COVID-19 pandemic caused >1 million infections during January-March 2020. There is an urgent need for reliable antibody detection approaches to support diagnosis, vaccine development, safe release of individuals from quarantine, and population lock-down exit strategies. We set out to evaluate the performance of ELISA and lateral flow immunoassay (LFIA) devices. Methods: We tested plasma for COVID (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2) IgM and IgG antibodies by ELISA and using nine different LFIA devices. We used a panel of plasma samples from individuals who have had confirmed COVID infection based on a PCR result (n=40), and pre-pandemic negative control samples banked in the UK prior to December-2019 (n=142). Results: ELISA detected IgM or IgG in 34/40 individuals with a confirmed history of COVID infection (sensitivity 85%, 95%CI 70-94%), vs. 0/50 pre-pandemic controls (specificity 100% [95%CI 93-100%]). IgG levels were detected in 31/31 COVID-positive individuals tested >/=10 days after symptom onset (sensitivity 100%, 95%CI 89-100%). IgG titres rose during the 3 weeks post symptom onset and began to fall by 8 weeks, but remained above the detection threshold. Point estimates for the sensitivity of LFIA devices ranged from 55-70% versus RT-PCR and 65-85% versus ELISA, with specificity 95-100% and 93-100% respectively. Within the limits of the study size, the performance of most LFIA devices was similar. Conclusions: Currently available commercial LFIA devices do not perform sufficiently well for individual patient applications. However, ELISA can be calibrated to be specific for detecting and quantifying SARS-CoV-2 IgM and IgG and is highly sensitive for IgG from 10 days following first symptoms.
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Wellcome+Open+Res 2020 ; 5 (ä): 139
