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10.3389/fgene.2021.600111 http://scihub22266oqcxt.onion/10.3389/fgene.2021.600111 33747038!7973286!33747038     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Genet 2021 ; 12 (ä): 600111 Nephropedia Template TP {{tp|p=33747038|t=2021. Toward Accurate and Robust Environmental Surveillance Using Metagenomics |pdf=|usr=}}{{33747038}} gab.com Text Toward Accurate and Robust Environmental Surveillance Using Metagenomics JO: Front Genet http://www.kidney.de/pget.php?&tw=1&pmid=33747038 #FOAvip Environmental surveillance is a critical tool for combatting public health threats represented by the global COVID-19 pandemic and the continuous increase of antibiotic resistance in pathogens. With its power to detect entire microbial communities, metagenomics-based methods stand out in addressing the need. However, several hurdles remain to be overcome in order to generate actionable interpretations from metagenomic sequencing data for infection prevention. Conceptually and technically, we focus on viability assessment, taxonomic resolution, and quantitative metagenomics, and discuss their current advancements, necessary precautions and directions to further development. We highlight the importance of building solid conceptual frameworks and identifying rational limits to facilitate the application of techniques. We also propose the usage of internal standards as a promising approach to overcome analytical bottlenecks introduced by low biomass samples and the inherent lack of quantitation in metagenomics. Taken together, we hope this perspective will contribute to bringing accurate and consistent metagenomics-based environmental surveillance to the ground. Twit Text FOAVip Toward Accurate and Robust Environmental Surveillance Using Metagenomics ????Front Genet http://www.kidney.de/pget.php?&tw=1&pmid=33747038 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33747038 #FOAvip English Wikipedia <ref>{{Cite pmid|33747038}}</ref> Toward Accurate and Robust Environmental Surveillance Using Metagenomics #MMPMID33747038 Shen J; McFarland AG; Young VB; Hayden MK; Hartmann EM Front Genet 2021[]; 12 (ä): 600111 PMID33747038show ga Environmental surveillance is a critical tool for combatting public health threats represented by the global COVID-19 pandemic and the continuous increase of antibiotic resistance in pathogens. With its power to detect entire microbial communities, metagenomics-based methods stand out in addressing the need. However, several hurdles remain to be overcome in order to generate actionable interpretations from metagenomic sequencing data for infection prevention. Conceptually and technically, we focus on viability assessment, taxonomic resolution, and quantitative metagenomics, and discuss their current advancements, necessary precautions and directions to further development. We highlight the importance of building solid conceptual frameworks and identifying rational limits to facilitate the application of techniques. We also propose the usage of internal standards as a promising approach to overcome analytical bottlenecks introduced by low biomass samples and the inherent lack of quantitation in metagenomics. Taken together, we hope this perspective will contribute to bringing accurate and consistent metagenomics-based environmental surveillance to the ground. äToward Accurate and Robust Environmental Surveillance Using Metagenomi(epmc).pdf DeepDyve Pubget Overpricing