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10.1016/j.jviromet.2021.114129 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114129 33745966!7970791!33745966     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 292 (ä): 114129 Nephropedia Template TP {{tp|p=33745966|t=2021. Usefulness of a multiplex immunodot in case of discordant results between automated COVID-19 serological assays |pdf=|usr=}}{{33745966}} gab.com Text Usefulness of a multiplex immunodot in case of discordant results between automated COVID-19 serological assays JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33745966 #FOAvip BACKGROUND: At present, the only reliable test for COVID-19 diagnosis is RT-qPCR. Serological assays have been widely used to increase the detection sensitivity of infected population. Hereby, we report the performance of a new pan-IgG multiplex Enzyme Immunoassay (immunodot) method for exploration of discrepant SARS-COV-2 serological results. METHODS: A retrospective study on 38 residual serum samples from recovered COVID-19 subjects with discordant serological results on Roche and Snibe platforms, were reanalyzed on a new semi-automated pan-IgG immunodot Enzyme Immunoassay, namely COVIDOT-TEST, in order to find the source of discrepancies and to evaluate the latter method. All samples were analyzed on the BlueDiver(R) Instrument and all strips were read by the BlueScan(R) Scanner using Dr DOT(R) Software. RESULTS: Based on our data, subject samples showed specific IgG reactions on >/= 2 different antigens on immunodot strips. Of these 38 samples, 97.4 % of samples showed specific IgG reaction against S1 + S2 antigens, 89.5 % showed against RBD antigen, 86.8 % against S2 antigen reaction on the COVIDOT-TEST kit. Specific IgG-S1 antigen and IgG-N antigen reactions were detected in 73.7 % and 65.8 % of the samples, respectively. CONCLUSION: The new semi-automated pan-IgG immunodot Enzyme Immunoassay method appeared to be a reliable assay to confirm suspicious COVID-19 serological screening results. Twit Text FOAVip Usefulness of a multiplex immunodot in case of discordant results between automated COVID-19 serological assays ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33745966 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33745966 #FOAvip English Wikipedia <ref>{{Cite pmid|33745966}}</ref> Usefulness of a multiplex immunodot in case of discordant results between automated COVID-19 serological assays #MMPMID33745966 Soleimani R; Khourssaji M; Aupaix A; Rodriguez-Villalobos H; Scohy A; Kabamba-Mukadi B J Virol Methods 2021[Jun]; 292 (ä): 114129 PMID33745966show ga BACKGROUND: At present, the only reliable test for COVID-19 diagnosis is RT-qPCR. Serological assays have been widely used to increase the detection sensitivity of infected population. Hereby, we report the performance of a new pan-IgG multiplex Enzyme Immunoassay (immunodot) method for exploration of discrepant SARS-COV-2 serological results. METHODS: A retrospective study on 38 residual serum samples from recovered COVID-19 subjects with discordant serological results on Roche and Snibe platforms, were reanalyzed on a new semi-automated pan-IgG immunodot Enzyme Immunoassay, namely COVIDOT-TEST, in order to find the source of discrepancies and to evaluate the latter method. All samples were analyzed on the BlueDiver(R) Instrument and all strips were read by the BlueScan(R) Scanner using Dr DOT(R) Software. RESULTS: Based on our data, subject samples showed specific IgG reactions on >/= 2 different antigens on immunodot strips. Of these 38 samples, 97.4 % of samples showed specific IgG reaction against S1 + S2 antigens, 89.5 % showed against RBD antigen, 86.8 % against S2 antigen reaction on the COVIDOT-TEST kit. Specific IgG-S1 antigen and IgG-N antigen reactions were detected in 73.7 % and 65.8 % of the samples, respectively. CONCLUSION: The new semi-automated pan-IgG immunodot Enzyme Immunoassay method appeared to be a reliable assay to confirm suspicious COVID-19 serological screening results. |Adult[MESH] |Antibodies, Viral/blood[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Female[MESH] |Humans[MESH] |Immunoenzyme Techniques/*methods[MESH] |Immunoglobulin G/blood[MESH] |Male[MESH] |Retrospective Studies[MESH]Usefulness of a multiplex immunodot in case of discordant results betw(epmc).pdf DeepDyve Pubget Overpricing