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10.1016/j.jbiosc.2021.02.006

http://scihub22266oqcxt.onion/10.1016/j.jbiosc.2021.02.006
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33745778!7972868!33745778
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suck abstract from ncbi


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pmid33745778      J+Biosci+Bioeng 2021 ; 131 (6): 696-702
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  • Rapid and reliable hybridoma screening method that is suitable for production of functional structure-recognizing monoclonal antibody #MMPMID33745778
  • Sakaguchi A; Nakajima C; Sawano A; Tanaka Y; Kurihara Y
  • J Biosci Bioeng 2021[Jun]; 131 (6): 696-702 PMID33745778show ga
  • Monoclonal antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in antibody drugs and test drugs. There is also a strong need to develop high-quality neutralizing antibodies as soon as possible in order to stop the rapid spread of new infectious diseases such as the SARS-CoV-2 virus. This study has developed a membrane-type immunoglobulin-directed hybridoma screening (MIHS) method for obtaining high-quality monoclonal antibodies with high efficiency and high speed. In addition to these advantages, this paper demonstrates that the MIHS method can selectively obtain monoclonal antibodies that specifically recognize the functional structure of proteins. The MIHS method is a useful technology that greatly contributes to the research community because it can be easily introduced in any laboratory that uses a flow cytometer.
  • |Animals[MESH]
  • |Antibodies, Monoclonal/*analysis/biosynthesis/*immunology/isolation & purification[MESH]
  • |Antibodies, Neutralizing/analysis/biosynthesis/immunology/isolation & purification[MESH]
  • |Antibody Specificity/*immunology[MESH]
  • |Cell Line, Tumor[MESH]
  • |Enzyme-Linked Immunosorbent Assay/methods[MESH]
  • |Flow Cytometry/methods[MESH]
  • |Green Fluorescent Proteins/genetics/immunology/metabolism[MESH]
  • |Humans[MESH]
  • |Hybridomas/cytology/*metabolism[MESH]
  • |Immunoglobulin Isotypes[MESH]
  • |Immunoprecipitation[MESH]
  • |Mice[MESH]


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