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10.1016/j.jmb.2021.166946 http://scihub22266oqcxt.onion/10.1016/j.jmb.2021.166946 33744314!7969826!33744314     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Mol+Biol 2021 ; 433 (10): 166946 Nephropedia Template TP {{tp|p=33744314|t=2021. SARS-CoV-2 Fusion Peptide has a Greater Membrane Perturbating Effect than SARS-CoV with Highly Specific Dependence on Ca(2) |pdf=|usr=}}{{33744314}} gab.com Text SARS-CoV-2 Fusion Peptide has a Greater Membrane Perturbating Effect than SARS-CoV with Highly Specific Dependence on Ca(2) JO: J Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=33744314 #FOAvip Coronaviruses are a major infectious disease threat, and include the zoonotic-origin human pathogens SARS-CoV-2, SARS-CoV, and MERS-CoV (SARS-2, SARS-1, and MERS). Entry of coronaviruses into host cells is mediated by the spike (S) protein. In our previous ESR studies, the local membrane ordering effect of the fusion peptide (FP) of various viral glycoproteins including the S of SARS-1 and MERS has been consistently observed. We previously determined that the sequence immediately downstream from the S2' cleavage site is the bona fide SARS-1 FP. In this study, we used sequence alignment to identify the SARS-2 FP, and studied its membrane ordering effect. Although there are only three residue differences, SARS-2 FP induces even greater membrane ordering than SARS-1 FP, possibly due to its greater hydrophobicity. This may be a reason that SARS-2 is better able to infect host cells. In addition, the membrane binding enthalpy for SARS-2 is greater. Both the membrane ordering of SARS-2 and SARS-1 FPs are dependent on Ca(2+), but that of SARS-2 shows a greater response to the presence of Ca(2+). Both FPs bind two Ca(2+) ions as does SARS-1 FP, but the two Ca(2+) binding sites of SARS-2 exhibit greater cooperativity. This Ca(2+) dependence by the SARS-2 FP is very ion-specific. These results show that Ca(2+) is an important regulator that interacts with the SARS-2 FP and thus plays a significant role in SARS-2 viral entry. This could lead to therapeutic solutions that either target the FP-calcium interaction or block the Ca(2+) channel. Twit Text FOAVip SARS-CoV-2 Fusion Peptide has a Greater Membrane Perturbating Effect than SARS-CoV with Highly Specific Dependence on Ca(2) ????J Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=33744314 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33744314 #FOAvip English Wikipedia <ref>{{Cite pmid|33744314}}</ref> SARS-CoV-2 Fusion Peptide has a Greater Membrane Perturbating Effect than SARS-CoV with Highly Specific Dependence on Ca(2) #MMPMID33744314 Lai AL; Freed JH J Mol Biol 2021[May]; 433 (10): 166946 PMID33744314show ga Coronaviruses are a major infectious disease threat, and include the zoonotic-origin human pathogens SARS-CoV-2, SARS-CoV, and MERS-CoV (SARS-2, SARS-1, and MERS). Entry of coronaviruses into host cells is mediated by the spike (S) protein. In our previous ESR studies, the local membrane ordering effect of the fusion peptide (FP) of various viral glycoproteins including the S of SARS-1 and MERS has been consistently observed. We previously determined that the sequence immediately downstream from the S2' cleavage site is the bona fide SARS-1 FP. In this study, we used sequence alignment to identify the SARS-2 FP, and studied its membrane ordering effect. Although there are only three residue differences, SARS-2 FP induces even greater membrane ordering than SARS-1 FP, possibly due to its greater hydrophobicity. This may be a reason that SARS-2 is better able to infect host cells. In addition, the membrane binding enthalpy for SARS-2 is greater. Both the membrane ordering of SARS-2 and SARS-1 FPs are dependent on Ca(2+), but that of SARS-2 shows a greater response to the presence of Ca(2+). Both FPs bind two Ca(2+) ions as does SARS-1 FP, but the two Ca(2+) binding sites of SARS-2 exhibit greater cooperativity. This Ca(2+) dependence by the SARS-2 FP is very ion-specific. These results show that Ca(2+) is an important regulator that interacts with the SARS-2 FP and thus plays a significant role in SARS-2 viral entry. This could lead to therapeutic solutions that either target the FP-calcium interaction or block the Ca(2+) channel. |Amino Acid Sequence[MESH] |Binding Sites[MESH] |Calcium/*metabolism/pharmacology[MESH] |Calorimetry[MESH] |Cell Membrane/drug effects/*metabolism/virology[MESH] |Hydrogen-Ion Concentration[MESH] |Hydrophobic and Hydrophilic Interactions[MESH] |SARS-CoV-2/drug effects/*metabolism[MESH] |Severe acute respiratory syndrome-related coronavirus/drug effects/*metabolism[MESH] |Spike Glycoprotein, Coronavirus/chemistry/genetics/*metabolism[MESH] |Thermodynamics[MESH] |Viral Fusion Proteins/chemistry/genetics/*metabolism[MESH]SARS-CoV-2 Fusion Peptide has a Greater Membrane Perturbating Effect t(epmc).pdf DeepDyve Pubget Overpricing