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10.1038/s41467-021-21996-6 http://scihub22266oqcxt.onion/10.1038/s41467-021-21996-6 33741959!7979722!33741959     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 1739 Nephropedia Template TP {{tp|p=33741959|t=2021. An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing |pdf=|usr=}}{{33741959}} gab.com Text An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33741959 #FOAvip Extensive testing is essential to break the transmission of SARS-CoV-2, which causes the ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is robust to viral genome mutations and temperature, produces results fast, can be applied directly on nasopharyngeal (NP) specimens without RNA purification, and incorporates a human internal control within the same reaction. Specifically, we show that the use of an engineered AsCas12a enzyme enables detection of wildtype and mutated SARS-CoV-2 and allows us to perform the detection step with loop-mediated isothermal amplification (LAMP) at 60-65 degrees C. We also find that the use of hybrid DNA-RNA guides increases the rate of reaction, enabling our test to be completed within 30 minutes. Utilizing clinical samples from 72 patients with COVID-19 infection and 57 healthy individuals, we demonstrate that our test exhibits a specificity and positive predictive value of 100% with a sensitivity of 50 and 1000 copies per reaction (or 2 and 40 copies per microliter) for purified RNA samples and unpurified NP specimens respectively. Twit Text FOAVip An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33741959 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33741959 #FOAvip English Wikipedia <ref>{{Cite pmid|33741959}}</ref> An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing #MMPMID33741959 Ooi KH; Liu MM; Tay JWD; Teo SY; Kaewsapsak P; Jin S; Lee CK; Hou J; Maurer-Stroh S; Lin W; Yan B; Yan G; Gao YG; Tan MH Nat Commun 2021[Mar]; 12 (1): 1739 PMID33741959show ga Extensive testing is essential to break the transmission of SARS-CoV-2, which causes the ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is robust to viral genome mutations and temperature, produces results fast, can be applied directly on nasopharyngeal (NP) specimens without RNA purification, and incorporates a human internal control within the same reaction. Specifically, we show that the use of an engineered AsCas12a enzyme enables detection of wildtype and mutated SARS-CoV-2 and allows us to perform the detection step with loop-mediated isothermal amplification (LAMP) at 60-65 degrees C. We also find that the use of hybrid DNA-RNA guides increases the rate of reaction, enabling our test to be completed within 30 minutes. Utilizing clinical samples from 72 patients with COVID-19 infection and 57 healthy individuals, we demonstrate that our test exhibits a specificity and positive predictive value of 100% with a sensitivity of 50 and 1000 copies per reaction (or 2 and 40 copies per microliter) for purified RNA samples and unpurified NP specimens respectively. |*RNA, Guide, CRISPR-Cas Systems[MESH] |Bacterial Proteins/genetics[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |CRISPR-Associated Proteins/genetics[MESH] |CRISPR-Cas Systems[MESH] |Clustered Regularly Interspaced Short Palindromic Repeats[MESH] |Endodeoxyribonucleases/genetics[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/methods[MESH] |Mutation[MESH] |Nasopharynx/virology[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/*genetics[MESH]An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable r(epmc).pdf DeepDyve Pubget Overpricing