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10.1016/j.scitotenv.2021.146133 http://scihub22266oqcxt.onion/10.1016/j.scitotenv.2021.146133 33740558!9752560!33740558     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33740558&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Total+Environ 2021 ; 779 (ä): 146133 Nephropedia Template TP {{tp|p=33740558|t=2021. Prevalence and stability of SARS-CoV-2 RNA on Bangladeshi banknotes |pdf=|usr=}}{{33740558}} gab.com Text Prevalence and stability of SARS-CoV-2 RNA on Bangladeshi banknotes JO: Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=33740558 #FOAvip Originating in December 2019 in China, SARS-CoV-2 has emerged as the deadliest pandemic in humankind's history. Along with direct contact and droplet contaminations, the possibility of infections through contaminated surfaces and fomites is investigating. This study aims to assess SARS-CoV-2 viral RNA's prevalence by real-time one-step reverse transcriptase PCR on banknotes circulating in Bangladesh. We also evaluated the persistence of the virus on banknotes spiked with SARS-CoV-2 positive diluted human nasopharyngeal samples. Among the 425 banknote samples collected from different entities, 7.29% (n = 31) were tested positive for targeted genes. Twenty-four positive representative samples were assessed for n gene fragments by conventional PCR and sequenced. All the samples that carry viral RNA belonged to the GR clade, the predominantly circulating clade in Bangladesh. In the stability test, the n gene was detected for up to 72 h on banknotes spiked with nasopharyngeal samples, and CT values increase significantly with time (p < 0.05). orf1b gene was observed to be less stable, especially on old banknotes, and usually went beyond detectable limit within 8 to 10 h. The stability of virus RNA well fitted by the Weibull model and concave curve for new banknotes and convex curve for old banknotes revealed. Handling banknotes is unavoidable; hence, these findings imply that proper hygiene practice is needed to limit SARS-CoV-2 transmission through banknotes. Twit Text FOAVip Prevalence and stability of SARS-CoV-2 RNA on Bangladeshi banknotes ????Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=33740558 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33740558 #FOAvip English Wikipedia <ref>{{Cite pmid|33740558}}</ref> Prevalence and stability of SARS-CoV-2 RNA on Bangladeshi banknotes #MMPMID33740558 Akter S; Roy PC; Ferdaus A; Ibnat H; Alam ASMRU; Nigar S; Jahid IK; Hossain MA Sci Total Environ 2021[Jul]; 779 (ä): 146133 PMID33740558show ga Originating in December 2019 in China, SARS-CoV-2 has emerged as the deadliest pandemic in humankind's history. Along with direct contact and droplet contaminations, the possibility of infections through contaminated surfaces and fomites is investigating. This study aims to assess SARS-CoV-2 viral RNA's prevalence by real-time one-step reverse transcriptase PCR on banknotes circulating in Bangladesh. We also evaluated the persistence of the virus on banknotes spiked with SARS-CoV-2 positive diluted human nasopharyngeal samples. Among the 425 banknote samples collected from different entities, 7.29% (n = 31) were tested positive for targeted genes. Twenty-four positive representative samples were assessed for n gene fragments by conventional PCR and sequenced. All the samples that carry viral RNA belonged to the GR clade, the predominantly circulating clade in Bangladesh. In the stability test, the n gene was detected for up to 72 h on banknotes spiked with nasopharyngeal samples, and CT values increase significantly with time (p < 0.05). orf1b gene was observed to be less stable, especially on old banknotes, and usually went beyond detectable limit within 8 to 10 h. The stability of virus RNA well fitted by the Weibull model and concave curve for new banknotes and convex curve for old banknotes revealed. Handling banknotes is unavoidable; hence, these findings imply that proper hygiene practice is needed to limit SARS-CoV-2 transmission through banknotes. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Bangladesh/epidemiology[MESH] |China/epidemiology[MESH] |Humans[MESH] |Prevalence[MESH]Prevalence and stability of SARS-CoV-2 RNA on Bangladeshi banknotes (epmc).pdf DeepDyve Pubget Overpricing