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10.1016/j.aca.2021.338310 http://scihub22266oqcxt.onion/10.1016/j.aca.2021.338310 33736798!7877206!33736798     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Chim+Acta 2021 ; 1154 (ä): 338310 Nephropedia Template TP {{tp|p=33736798|t=2021. A novel One-pot rapid diagnostic technology for COVID-19 |pdf=|usr=}}{{33736798}} gab.com Text A novel One-pot rapid diagnostic technology for COVID-19 JO: Anal Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33736798 #FOAvip Novel coronavirus disease (COVID-19) caused by SARS-CoV-2 is an ongoing global pandemic associated with high rates of morbidity and mortality. RT-qPCR has become the diagnostic standard for the testing of SARS-CoV-2 in most countries. COVID-19 diagnosis generally relies upon RT-qPCR-mediated identification of SARS-CoV-2 viral RNA, which is costly, labor-extensive, and requires specialized training and equipment. Herein, we established a novel one-tube rapid diagnostic approach based upon formamide and colorimetric RT-LAMP (One-Pot RT-LAMP) that can be used to diagnose COVID-19 without the extraction of specific viral RNA. The technique could visually detect SARS-CoV-2 within 45 min with a limit of detection of 5 copies per reaction in extracted RNA, and about 7.66 virus copies per muL in viral transport medium. The One-Pot RT-LAMP test showed a high specificity without cross-reactivity with 12 viruses including SARS-CoV, MERS-CoV, and human infectious influenza virus (H1N1/H3N2 of influenza A and B virus, ect. We validated this One-Pot RT-LAMP approach by its successful use for the analysis of 45 clinical nasopharyngeal swab samples, yielding results identical to those of traditional RT-qPCR analyses, while achieving good selectivity and sensitivity relative to a commercial RT-qPCR approach. As such, this One-Pot RT-LAMP technology may be a valid means of conducting high-sensitivity, low-cost and rapid SARS-CoV-2 identification without the extraction of viral RNA. Twit Text FOAVip A novel One-pot rapid diagnostic technology for COVID-19 ????Anal Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33736798 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33736798 #FOAvip English Wikipedia <ref>{{Cite pmid|33736798}}</ref> A novel One-pot rapid diagnostic technology for COVID-19 #MMPMID33736798 Li J; Hu X; Wang X; Yang J; Zhang L; Deng Q; Zhang X; Wang Z; Hou T; Li S Anal Chim Acta 2021[Apr]; 1154 (ä): 338310 PMID33736798show ga Novel coronavirus disease (COVID-19) caused by SARS-CoV-2 is an ongoing global pandemic associated with high rates of morbidity and mortality. RT-qPCR has become the diagnostic standard for the testing of SARS-CoV-2 in most countries. COVID-19 diagnosis generally relies upon RT-qPCR-mediated identification of SARS-CoV-2 viral RNA, which is costly, labor-extensive, and requires specialized training and equipment. Herein, we established a novel one-tube rapid diagnostic approach based upon formamide and colorimetric RT-LAMP (One-Pot RT-LAMP) that can be used to diagnose COVID-19 without the extraction of specific viral RNA. The technique could visually detect SARS-CoV-2 within 45 min with a limit of detection of 5 copies per reaction in extracted RNA, and about 7.66 virus copies per muL in viral transport medium. The One-Pot RT-LAMP test showed a high specificity without cross-reactivity with 12 viruses including SARS-CoV, MERS-CoV, and human infectious influenza virus (H1N1/H3N2 of influenza A and B virus, ect. We validated this One-Pot RT-LAMP approach by its successful use for the analysis of 45 clinical nasopharyngeal swab samples, yielding results identical to those of traditional RT-qPCR analyses, while achieving good selectivity and sensitivity relative to a commercial RT-qPCR approach. As such, this One-Pot RT-LAMP technology may be a valid means of conducting high-sensitivity, low-cost and rapid SARS-CoV-2 identification without the extraction of viral RNA. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |DNA Primers/chemistry/metabolism[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques[MESH] |Nasopharynx/virology[MESH] |Nucleic Acid Amplification Techniques[MESH] |RNA, Viral/analysis/metabolism[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2/genetics/isolation & purification[MESH]A novel One-pot rapid diagnostic technology for COVID-19 (epmc).pdf DeepDyve Pubget Overpricing