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10.1111/vox.13056 http://scihub22266oqcxt.onion/10.1111/vox.13056 33734455!8250394!33734455     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Vox+Sang 2021 ; 116 (6): 665-672 Nephropedia Template TP {{tp|p=33734455|t=2021. Impact of pathogen reduction methods on immunological properties of the COVID-19 convalescent plasma |pdf=|usr=}}{{33734455}} gab.com Text Impact of pathogen reduction methods on immunological properties of the COVID-19 convalescent plasma JO: Vox Sang http://www.kidney.de/pget.php?&tw=1&pmid=33734455 #FOAvip BACKGROUND AND OBJECTIVES: COVID-19 convalescent plasma is an experimental treatment against SARS-CoV-2. The aim of this study is to assess the impact of different pathogen reduction methods on the levels and virus neutralizing activity of the specific antibodies against SARS-CoV2 in convalescent plasma. MATERIALS AND METHODS: A total of 140 plasma doses collected by plasmapheresis from COVID-19 convalescent donors were subjected to pathogen reduction by three methods: methylene blue (M)/visible light, riboflavin (R)/UVB and amotosalen (A)/UVA. To conduct a paired comparison, individual plasma doses were divided into 2 samples that were subjected to one of these methods. The titres of SARS-CoV2 neutralizing antibodies (NtAbs) and levels of specific immunoglobulins to RBD, S- and N-proteins of SARS-CoV-2 were measured before and after pathogen reduction. RESULTS: The methods reduced NtAbs titres differently: among units with the initial titre 80 or above, 81% of units remained unchanged and 19% decreased by one step after methylene blue; 60% were unchanged and 40% decreased by one step after amotosalen; after riboflavin 43% were unchanged and 50% (7%, respectively) had a one-step (two-step, respectively) decrease. Paired two-sample comparisons (M vs. A, M vs. R and A vs. R) revealed that the largest statistically significant decrease in quantity and activity of the specific antibodies resulted from the riboflavin treatment. CONCLUSION: Pathogen reduction with methylene blue or with amotosalen provides the greater likelihood of preserving the immunological properties of the COVID-19 convalescent plasma compared to riboflavin. Twit Text FOAVip Impact of pathogen reduction methods on immunological properties of the COVID-19 convalescent plasma ????Vox Sang http://www.kidney.de/pget.php?&tw=1&pmid=33734455 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33734455 #FOAvip English Wikipedia <ref>{{Cite pmid|33734455}}</ref> Impact of pathogen reduction methods on immunological properties of the COVID-19 convalescent plasma #MMPMID33734455 Kostin AI; Lundgren MN; Bulanov AY; Ladygina EA; Chirkova KS; Gintsburg AL; Logunov DY; Dolzhikova IV; Shcheblyakov DV; Borovkova NV; Godkov MA; Bazhenov AI; Shustov VV; Bogdanova AS; Kamalova AR; Ganchin VV; Dombrovskiy EA; Volkov SE; Drozdova NE; Petrikov SS Vox Sang 2021[Jul]; 116 (6): 665-672 PMID33734455show ga BACKGROUND AND OBJECTIVES: COVID-19 convalescent plasma is an experimental treatment against SARS-CoV-2. The aim of this study is to assess the impact of different pathogen reduction methods on the levels and virus neutralizing activity of the specific antibodies against SARS-CoV2 in convalescent plasma. MATERIALS AND METHODS: A total of 140 plasma doses collected by plasmapheresis from COVID-19 convalescent donors were subjected to pathogen reduction by three methods: methylene blue (M)/visible light, riboflavin (R)/UVB and amotosalen (A)/UVA. To conduct a paired comparison, individual plasma doses were divided into 2 samples that were subjected to one of these methods. The titres of SARS-CoV2 neutralizing antibodies (NtAbs) and levels of specific immunoglobulins to RBD, S- and N-proteins of SARS-CoV-2 were measured before and after pathogen reduction. RESULTS: The methods reduced NtAbs titres differently: among units with the initial titre 80 or above, 81% of units remained unchanged and 19% decreased by one step after methylene blue; 60% were unchanged and 40% decreased by one step after amotosalen; after riboflavin 43% were unchanged and 50% (7%, respectively) had a one-step (two-step, respectively) decrease. Paired two-sample comparisons (M vs. A, M vs. R and A vs. R) revealed that the largest statistically significant decrease in quantity and activity of the specific antibodies resulted from the riboflavin treatment. CONCLUSION: Pathogen reduction with methylene blue or with amotosalen provides the greater likelihood of preserving the immunological properties of the COVID-19 convalescent plasma compared to riboflavin. |Antibodies, Neutralizing/blood[MESH] |Blood Safety/*methods[MESH] |Blood-Borne Pathogens/*isolation & purification[MESH] |COVID-19 Serotherapy[MESH] |COVID-19/immunology/*therapy[MESH] |Furocoumarins[MESH] |Humans[MESH] |Immunization, Passive[MESH] |Methylene Blue[MESH] |Plasma/*immunology[MESH] |Riboflavin[MESH]Impact of pathogen reduction methods on immunological properties of th(epmc).pdf DeepDyve Pubget Overpricing