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10.1126/sciadv.abe5940 http://scihub22266oqcxt.onion/10.1126/sciadv.abe5940 33731349!7968834!33731349     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Adv 2021 ; 7 (12): ä Nephropedia Template TP {{tp|p=33731349|t=2021. Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2 |pdf=|usr=}}{{33731349}} gab.com Text Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2 JO: Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33731349 #FOAvip Despite the importance of nucleic acid testing in managing the COVID-19 pandemic, current detection approaches remain limited due to their high complexity and extensive processing. Here, we describe a molecular nanotechnology that enables direct and sensitive detection of viral RNA targets in native clinical samples. The technology, termed catalytic amplification by transition-state molecular switch (CATCH), leverages DNA-enzyme hybrid complexes to form a molecular switch. By ratiometric tuning of its constituents, the multicomponent molecular switch is prepared in a hyperresponsive state-the transition state-that can be readily activated upon the binding of sparse RNA targets to turn on substantial enzymatic activity. CATCH thus achieves superior performance (~8 RNA copies/mul), direct fluorescence detection that bypasses all steps of PCR (<1 hour at room temperature), and versatile implementation (high-throughput 96-well format and portable microfluidic assay). When applied for clinical COVID-19 diagnostics, CATCH demonstrated direct and accurate detection in minimally processed patient swab samples. Twit Text FOAVip Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2 ????Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33731349 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33731349 #FOAvip English Wikipedia <ref>{{Cite pmid|33731349}}</ref> Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2 #MMPMID33731349 Sundah NR; Natalia A; Liu Y; Ho NRY; Zhao H; Chen Y; Miow QH; Wang Y; Beh DLL; Chew KL; Chan D; Tambyah PA; Ong CWM; Shao H Sci Adv 2021[Mar]; 7 (12): ä PMID33731349show ga Despite the importance of nucleic acid testing in managing the COVID-19 pandemic, current detection approaches remain limited due to their high complexity and extensive processing. Here, we describe a molecular nanotechnology that enables direct and sensitive detection of viral RNA targets in native clinical samples. The technology, termed catalytic amplification by transition-state molecular switch (CATCH), leverages DNA-enzyme hybrid complexes to form a molecular switch. By ratiometric tuning of its constituents, the multicomponent molecular switch is prepared in a hyperresponsive state-the transition state-that can be readily activated upon the binding of sparse RNA targets to turn on substantial enzymatic activity. CATCH thus achieves superior performance (~8 RNA copies/mul), direct fluorescence detection that bypasses all steps of PCR (<1 hour at room temperature), and versatile implementation (high-throughput 96-well format and portable microfluidic assay). When applied for clinical COVID-19 diagnostics, CATCH demonstrated direct and accurate detection in minimally processed patient swab samples. |*COVID-19 Nucleic Acid Testing/instrumentation/methods[MESH] |*COVID-19/diagnosis/genetics[MESH] |*Lab-On-A-Chip Devices[MESH] |*Microfluidic Analytical Techniques[MESH] |*Point-of-Care Testing[MESH] |Humans[MESH] |Limit of Detection[MESH]Catalytic amplification by transition-state molecular switches for dir(epmc).pdf DeepDyve Pubget Overpricing