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10.1371/journal.pbio.3001143 http://scihub22266oqcxt.onion/10.1371/journal.pbio.3001143 33730024!8007021!33730024     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33730024&cmd=llinks): Failed to open stream: HTTP request failed! 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Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes |pdf=|usr=}}{{33730024}} gab.com Text Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes JO: PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=33730024 #FOAvip There are currently limited Food and Drug Administration (FDA)-approved drugs and vaccines for the treatment or prevention of Coronavirus Disease 2019 (COVID-19). Enhanced understanding of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and pathogenesis is critical for the development of therapeutics. To provide insight into viral replication, cell tropism, and host-viral interactions of SARS-CoV-2, we performed single-cell (sc) RNA sequencing (RNA-seq) of experimentally infected human bronchial epithelial cells (HBECs) in air-liquid interface (ALI) cultures over a time course. This revealed novel polyadenylated viral transcripts and highlighted ciliated cells as a major target at the onset of infection, which we confirmed by electron and immunofluorescence microscopy. Over the course of infection, the cell tropism of SARS-CoV-2 expands to other epithelial cell types including basal and club cells. Infection induces cell-intrinsic expression of type I and type III interferons (IFNs) and interleukin (IL)-6 but not IL-1. This results in expression of interferon-stimulated genes (ISGs) in both infected and bystander cells. This provides a detailed characterization of genes, cell types, and cell state changes associated with SARS-CoV-2 infection in the human airway. Twit Text FOAVip Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes ????PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=33730024 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33730024 #FOAvip English Wikipedia <ref>{{Cite pmid|33730024}}</ref> Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes #MMPMID33730024 Ravindra NG; Alfajaro MM; Gasque V; Huston NC; Wan H; Szigeti-Buck K; Yasumoto Y; Greaney AM; Habet V; Chow RD; Chen JS; Wei J; Filler RB; Wang B; Wang G; Niklason LE; Montgomery RR; Eisenbarth SC; Chen S; Williams A; Iwasaki A; Horvath TL; Foxman EF; Pierce RW; Pyle AM; van Dijk D; Wilen CB PLoS Biol 2021[Mar]; 19 (3): e3001143 PMID33730024show ga There are currently limited Food and Drug Administration (FDA)-approved drugs and vaccines for the treatment or prevention of Coronavirus Disease 2019 (COVID-19). Enhanced understanding of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and pathogenesis is critical for the development of therapeutics. To provide insight into viral replication, cell tropism, and host-viral interactions of SARS-CoV-2, we performed single-cell (sc) RNA sequencing (RNA-seq) of experimentally infected human bronchial epithelial cells (HBECs) in air-liquid interface (ALI) cultures over a time course. This revealed novel polyadenylated viral transcripts and highlighted ciliated cells as a major target at the onset of infection, which we confirmed by electron and immunofluorescence microscopy. Over the course of infection, the cell tropism of SARS-CoV-2 expands to other epithelial cell types including basal and club cells. Infection induces cell-intrinsic expression of type I and type III interferons (IFNs) and interleukin (IL)-6 but not IL-1. This results in expression of interferon-stimulated genes (ISGs) in both infected and bystander cells. This provides a detailed characterization of genes, cell types, and cell state changes associated with SARS-CoV-2 infection in the human airway. |*Gene Expression[MESH] |Adult[MESH] |Bronchi/*pathology/virology[MESH] |COVID-19/*diagnosis/immunology/pathology/virology[MESH] |Cells, Cultured[MESH] |Epithelium/pathology/virology[MESH] |Humans[MESH] |Immunity, Innate[MESH] |Longitudinal Studies[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH] |Single-Cell Analysis/*methods[MESH] |Transcriptome[MESH]Single-cell longitudinal analysis of SARS-CoV-2 infection in human air(epmc).pdf DeepDyve Pubget Overpricing