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10.1371/journal.pone.0248061 http://scihub22266oqcxt.onion/10.1371/journal.pone.0248061 33730022!7968690!33730022     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2021 ; 16 (3): e0248061 Nephropedia Template TP {{tp|p=33730022|t=2021. Immuno-informatics design of a multimeric epitope peptide based vaccine targeting SARS-CoV-2 spike glycoprotein |pdf=|usr=}}{{33730022}} gab.com Text Immuno-informatics design of a multimeric epitope peptide based vaccine targeting SARS-CoV-2 spike glycoprotein JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33730022 #FOAvip Developing an efficacious vaccine for SARS-CoV-2 infection is critical to stemming COVID-19 fatalities and providing the global community with immune protection. We have used a bioinformatic approach to aid in designing an epitope peptide-based vaccine against the spike protein of the virus. Five antigenic B cell epitopes with viable antigenicity and a total of 27 discontinuous B cell epitopes were mapped out structurally in the spike protein for antibody recognition. We identified eight CD8+ T cell 9-mers and 12 CD4+ T cell 14-15-mer as promising candidate epitopes putatively restricted by a large number of MHC I and II alleles, respectively. We used this information to construct an in silico chimeric peptide vaccine whose translational rate was highly expressed when cloned in pET28a (+) vector. With our In silico test, the vaccine construct was predicted to elicit high antigenicity and cell-mediated immunity when given as a homologous prime-boost, triggering of toll-like receptor 5 by the adjuvant linker. The vaccine was also characterized by an increase in IgM and IgG and an array of Th1 and Th2 cytokines. Upon in silico challenge with SARS-CoV-2, there was a decrease in antigen levels using our immune simulations. We, therefore, propose that potential vaccine designs consider this approach. Twit Text FOAVip Immuno-informatics design of a multimeric epitope peptide based vaccine targeting SARS-CoV-2 spike glycoprotein ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33730022 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33730022 #FOAvip English Wikipedia <ref>{{Cite pmid|33730022}}</ref> Immuno-informatics design of a multimeric epitope peptide based vaccine targeting SARS-CoV-2 spike glycoprotein #MMPMID33730022 Chukwudozie OS; Gray CM; Fagbayi TA; Chukwuanukwu RC; Oyebanji VO; Bankole TT; Adewole RA; Daniel EM PLoS One 2021[]; 16 (3): e0248061 PMID33730022show ga Developing an efficacious vaccine for SARS-CoV-2 infection is critical to stemming COVID-19 fatalities and providing the global community with immune protection. We have used a bioinformatic approach to aid in designing an epitope peptide-based vaccine against the spike protein of the virus. Five antigenic B cell epitopes with viable antigenicity and a total of 27 discontinuous B cell epitopes were mapped out structurally in the spike protein for antibody recognition. We identified eight CD8+ T cell 9-mers and 12 CD4+ T cell 14-15-mer as promising candidate epitopes putatively restricted by a large number of MHC I and II alleles, respectively. We used this information to construct an in silico chimeric peptide vaccine whose translational rate was highly expressed when cloned in pET28a (+) vector. With our In silico test, the vaccine construct was predicted to elicit high antigenicity and cell-mediated immunity when given as a homologous prime-boost, triggering of toll-like receptor 5 by the adjuvant linker. The vaccine was also characterized by an increase in IgM and IgG and an array of Th1 and Th2 cytokines. Upon in silico challenge with SARS-CoV-2, there was a decrease in antigen levels using our immune simulations. We, therefore, propose that potential vaccine designs consider this approach. |CD4-Positive T-Lymphocytes/immunology[MESH] |CD8-Positive T-Lymphocytes/immunology[MESH] |COVID-19 Vaccines/*immunology[MESH] |COVID-19/immunology[MESH] |Computational Biology/methods[MESH] |Cytokines/immunology[MESH] |Epitopes, B-Lymphocyte/immunology[MESH] |Epitopes, T-Lymphocyte/*immunology[MESH] |Histocompatibility Antigens Class I/immunology[MESH] |Histocompatibility Antigens Class II/immunology[MESH] |Humans[MESH] |Immunity, Cellular/immunology[MESH] |Immunogenicity, Vaccine[MESH] |Immunoglobulin G/immunology[MESH] |Immunoglobulin M/immunology[MESH] |Molecular Docking Simulation[MESH] |Peptides/immunology[MESH] |SARS-CoV-2/*immunology[MESH] |Spike Glycoprotein, Coronavirus/*immunology[MESH]Immuno-informatics design of a multimeric epitope peptide based vaccin(epmc).pdf DeepDyve Pubget Overpricing