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10.1186/s13046-021-01876-z http://scihub22266oqcxt.onion/10.1186/s13046-021-01876-z 33726758!7968185!33726758     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Exp+Clin+Cancer+Res 2021 ; 40 (1): 100 Nephropedia Template TP {{tp|p=33726758|t=2021. O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination |pdf=|usr=}}{{33726758}} gab.com Text O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination JO: J Exp Clin Cancer Res http://www.kidney.de/pget.php?&tw=1&pmid=33726758 #FOAvip BACKGROUND: Multiple myeloma (MM) cell motility is a critical step during MM dissemination throughout the body, but how it is regulated remains largely unknown. As hypercalcemia is an important clinical feature of MM, high calcium (Ca(2+)) and altered Ca(2+) signaling could be a key contributing factor to the pathological process. METHODS: Bioinformatics analyses were employed to assess the clinical significance of Ca(2+) influx channels in clinical specimens of smoldering and symptomatic MM. Functional and regulatory roles of influx channels and downstream signaling in MM cell migration and invasion were conducted and experimental MM dissemination was examined in a xenograft mouse model using in vivo live imaging and engraftment analysis. RESULTS: Inhibition of TRPM7, ORAI1, and STIM1 influx channels, which are highly expressed in MM patients, and subsequent blockage of Ca(2+) influx by CRISPR/Cas9 and small molecule inhibitors, effectively inhibit MM cell migration and invasion, and attenuate the experimental MM dissemination. Mechanistic studies reveal a nutrient sensor O-GlcNAcylation as a downstream regulator of Ca(2+) influx that specifically targets cell adhesion molecules. Hyper-O-GlcNAcylation following the inhibition of Ca(2+) influx channels induces integrin alpha4 and integrin beta7 downregulation via ubiquitin-proteasomal degradation and represses the aggressive MM phenotype. CONCLUSIONS: Our findings unveil a novel regulatory mechanism of MM cell motility via Ca(2+) influx/O-GlcNAcylation axis that directly targets integrin alpha4 and integrin beta7, providing mechanistic insights into the pathogenesis and progression of MM and demonstrating potential predictive biomarkers and therapeutic targets for advanced MM. Twit Text FOAVip O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination ????J Exp Clin Cancer Res http://www.kidney.de/pget.php?&tw=1&pmid=33726758 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33726758 #FOAvip English Wikipedia <ref>{{Cite pmid|33726758}}</ref> O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination #MMPMID33726758 Samart P; Luanpitpong S; Rojanasakul Y; Issaragrisil S J Exp Clin Cancer Res 2021[Mar]; 40 (1): 100 PMID33726758show ga BACKGROUND: Multiple myeloma (MM) cell motility is a critical step during MM dissemination throughout the body, but how it is regulated remains largely unknown. As hypercalcemia is an important clinical feature of MM, high calcium (Ca(2+)) and altered Ca(2+) signaling could be a key contributing factor to the pathological process. METHODS: Bioinformatics analyses were employed to assess the clinical significance of Ca(2+) influx channels in clinical specimens of smoldering and symptomatic MM. Functional and regulatory roles of influx channels and downstream signaling in MM cell migration and invasion were conducted and experimental MM dissemination was examined in a xenograft mouse model using in vivo live imaging and engraftment analysis. RESULTS: Inhibition of TRPM7, ORAI1, and STIM1 influx channels, which are highly expressed in MM patients, and subsequent blockage of Ca(2+) influx by CRISPR/Cas9 and small molecule inhibitors, effectively inhibit MM cell migration and invasion, and attenuate the experimental MM dissemination. Mechanistic studies reveal a nutrient sensor O-GlcNAcylation as a downstream regulator of Ca(2+) influx that specifically targets cell adhesion molecules. Hyper-O-GlcNAcylation following the inhibition of Ca(2+) influx channels induces integrin alpha4 and integrin beta7 downregulation via ubiquitin-proteasomal degradation and represses the aggressive MM phenotype. CONCLUSIONS: Our findings unveil a novel regulatory mechanism of MM cell motility via Ca(2+) influx/O-GlcNAcylation axis that directly targets integrin alpha4 and integrin beta7, providing mechanistic insights into the pathogenesis and progression of MM and demonstrating potential predictive biomarkers and therapeutic targets for advanced MM. |Animals[MESH] |Calcium Channels/*metabolism[MESH] |Homeostasis/*genetics[MESH] |Humans[MESH] |Male[MESH] |Mice[MESH] |Multiple Myeloma/*genetics[MESH] |N-Acetylglucosaminyltransferases/*metabolism[MESH]O-GlcNAcylation homeostasis controlled by calcium influx channels regu(epmc).pdf DeepDyve Pubget Overpricing