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10.1007/s00705-021-04986-3 http://scihub22266oqcxt.onion/10.1007/s00705-021-04986-3 33723631!7959303!33723631     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Arch+Virol 2021 ; 166 (5): 1385-1393 Nephropedia Template TP {{tp|p=33723631|t=2021. Transmission of SARS-CoV-2 in northern Ghana: insights from whole-genome sequencing |pdf=|usr=}}{{33723631}} gab.com Text Transmission of SARS-CoV-2 in northern Ghana: insights from whole-genome sequencing JO: Arch Virol http://www.kidney.de/pget.php?&tw=1&pmid=33723631 #FOAvip Following the detection of the first imported case of COVID-19 in the northern sector of Ghana, we molecularly characterized and phylogenetically analysed sequences, including three complete genome sequences, of severe acute respiratory syndrome coronavirus 2 obtained from nine patients in Ghana. We performed high-throughput sequencing on nine samples that were found to have a high concentration of viral RNA. We also assessed the potential impact that long-distance transport of samples to testing centres may have on sequencing results. Here, two samples that were similar in terms of viral RNA concentration but were transported from sites that are over 400 km apart were analyzed. All sequences were compared to previous sequences from Ghana and representative sequences from regions where our patients had previously travelled. Three complete genome sequences and another nearly complete genome sequence with 95.6% coverage were obtained. Sequences with coverage in excess of 80% were found to belong to three lineages, namely A, B.1 and B.2. Our sequences clustered in two different clades, with the majority falling within a clade composed of sequences from sub-Saharan Africa. Less RNA fragmentation was seen in sample KATH23, which was collected 9 km from the testing site, than in sample TTH6, which was collected and transported over a distance of 400 km to the testing site. The clustering of several sequences from sub-Saharan Africa suggests regional circulation of the viruses in the subregion. Importantly, there may be a need to decentralize testing sites and build more capacity across Africa to boost the sequencing output of the subregion. Twit Text FOAVip Transmission of SARS-CoV-2 in northern Ghana: insights from whole-genome sequencing ????Arch Virol http://www.kidney.de/pget.php?&tw=1&pmid=33723631 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33723631 #FOAvip English Wikipedia <ref>{{Cite pmid|33723631}}</ref> Transmission of SARS-CoV-2 in northern Ghana: insights from whole-genome sequencing #MMPMID33723631 Sylverken AA; El-Duah P; Owusu M; Schneider J; Yeboah R; Ayisi-Boateng NK; Gorman R; Adu E; Kwarteng A; Frimpong M; Binger T; Aryeetey S; Asamoah JA; Amoako YA; Amuasi JH; Beheim-Schwarzbach J; Owusu-Dabo E; Adu-Sarkodie Y; Obiri-Danso K; Corman VM; Drosten C; Phillips R Arch Virol 2021[May]; 166 (5): 1385-1393 PMID33723631show ga Following the detection of the first imported case of COVID-19 in the northern sector of Ghana, we molecularly characterized and phylogenetically analysed sequences, including three complete genome sequences, of severe acute respiratory syndrome coronavirus 2 obtained from nine patients in Ghana. We performed high-throughput sequencing on nine samples that were found to have a high concentration of viral RNA. We also assessed the potential impact that long-distance transport of samples to testing centres may have on sequencing results. Here, two samples that were similar in terms of viral RNA concentration but were transported from sites that are over 400 km apart were analyzed. All sequences were compared to previous sequences from Ghana and representative sequences from regions where our patients had previously travelled. Three complete genome sequences and another nearly complete genome sequence with 95.6% coverage were obtained. Sequences with coverage in excess of 80% were found to belong to three lineages, namely A, B.1 and B.2. Our sequences clustered in two different clades, with the majority falling within a clade composed of sequences from sub-Saharan Africa. Less RNA fragmentation was seen in sample KATH23, which was collected 9 km from the testing site, than in sample TTH6, which was collected and transported over a distance of 400 km to the testing site. The clustering of several sequences from sub-Saharan Africa suggests regional circulation of the viruses in the subregion. Importantly, there may be a need to decentralize testing sites and build more capacity across Africa to boost the sequencing output of the subregion. |COVID-19/*transmission[MESH] |Female[MESH] |Genome, Viral[MESH] |Ghana[MESH] |Humans[MESH] |Male[MESH] |Nasopharynx/virology[MESH] |Oropharynx/virology[MESH] |Phylogeny[MESH] |SARS-CoV-2/*classification/genetics[MESH] |Sequence Analysis, RNA[MESH]Transmission of SARS-CoV-2 in northern Ghana: insights from whole-geno(epmc).pdf DeepDyve Pubget Overpricing