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10.1021/acsomega.1c00008 http://scihub22266oqcxt.onion/10.1021/acsomega.1c00008 33718731!7927290!33718731     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Omega 2021 ; 6 (9): 6404-6413 Nephropedia Template TP {{tp|p=33718731|t=2021. Optical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations |pdf=|usr=}}{{33718731}} gab.com Text Optical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations JO: ACS Omega http://www.kidney.de/pget.php?&tw=1&pmid=33718731 #FOAvip The emergence of a new strain of coronavirus in late 2019, SARS-CoV-2, led to a global pandemic in 2020. This may have been preventable if large scale, rapid diagnosis of active cases had been possible, and this has highlighted the need for more effective and efficient ways of detecting and managing viral infections. In this work, we investigate three different optical techniques for quantifying the binding of recombinant SARS-CoV-2 spike protein to surface-immobilized oligonucleotide aptamers. Biolayer interferometry is a relatively cheap, robust, and rapid method that only requires very small sample volumes. However, its detection limit of 250 nM means that it is not sensitive enough to detect antigen proteins at physiologically relevant levels (sub-pM). Surface plasmon resonance is a more sensitive technique but requires larger sample volumes, takes longer, requires more expensive instrumentation, and only reduces the detection limit to 5 nM. Surface-enhanced Raman spectroscopy is far more sensitive, enabling detection of spike protein to sub-picomolar concentrations. Control experiments performed using scrambled aptamers and using bovine serum albumin as an analyte show that this apta-sensing approach is both sensitive and selective, with no appreciable response observed for any controls. Overall, these proof-of-principle results demonstrate that SERS-based aptasensors hold great promise for development into rapid, point-of-use antigen detection systems, enabling mass testing without any need for reagents or laboratory expertise and equipment. Twit Text FOAVip Optical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations ????ACS Omega http://www.kidney.de/pget.php?&tw=1&pmid=33718731 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33718731 #FOAvip English Wikipedia <ref>{{Cite pmid|33718731}}</ref> Optical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations #MMPMID33718731 Stanborough T; Given FM; Koch B; Sheen CR; Stowers-Hull AB; Waterland MR; Crittenden DL ACS Omega 2021[Mar]; 6 (9): 6404-6413 PMID33718731show ga The emergence of a new strain of coronavirus in late 2019, SARS-CoV-2, led to a global pandemic in 2020. This may have been preventable if large scale, rapid diagnosis of active cases had been possible, and this has highlighted the need for more effective and efficient ways of detecting and managing viral infections. In this work, we investigate three different optical techniques for quantifying the binding of recombinant SARS-CoV-2 spike protein to surface-immobilized oligonucleotide aptamers. Biolayer interferometry is a relatively cheap, robust, and rapid method that only requires very small sample volumes. However, its detection limit of 250 nM means that it is not sensitive enough to detect antigen proteins at physiologically relevant levels (sub-pM). Surface plasmon resonance is a more sensitive technique but requires larger sample volumes, takes longer, requires more expensive instrumentation, and only reduces the detection limit to 5 nM. Surface-enhanced Raman spectroscopy is far more sensitive, enabling detection of spike protein to sub-picomolar concentrations. Control experiments performed using scrambled aptamers and using bovine serum albumin as an analyte show that this apta-sensing approach is both sensitive and selective, with no appreciable response observed for any controls. Overall, these proof-of-principle results demonstrate that SERS-based aptasensors hold great promise for development into rapid, point-of-use antigen detection systems, enabling mass testing without any need for reagents or laboratory expertise and equipment. äOptical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concen(epmc).pdf DeepDyve Pubget Overpricing