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10.3389/fmed.2021.617399

http://scihub22266oqcxt.onion/10.3389/fmed.2021.617399
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suck abstract from ncbi


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pmid33718401      Front+Med+(Lausanne) 2021 ; 8 (ä): 617399
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  • Performance of Saliva Samples for COVID-19 Diagnosis by Using the Allplex(TM) 2019-nCoV Assay Kit #MMPMID33718401
  • Tapia CV; Marcia C; Ivone M; Nadia P; Lesly M; Camila G; Valentina A; Paula I; Fabien M
  • Front Med (Lausanne) 2021[]; 8 (ä): 617399 PMID33718401show ga
  • Background: Although the nasopharyngeal swab (NPS) is the reference sampling method for the detection of SARS-Cov-2, it is not always possible to collect NPS in some patients. Saliva represents an interesting sampling method because it is less invasive and more convenient in patients with nasal or pharyngeal lesions. Objective: To compare the RT-qPCR test performances of saliva samples with nasal mid-turbinate swab (NMTS) and NPS samples in a cohort of ambulatory patients suspected of having COVID-19. Study Design: For each of the 112 enrolled patients, NPS, NMTS, and saliva samples were collected and tested for SARS-Cov-2 detection using three different target genes (RdRP, N and E genes) by RT-qPCR. Results: Among the positive samples (56/112), saliva samples showed a lower percentage of SARS-Cov-2 detection compared to NPS samples, (85.7 vs. 96.4%), while still a lower percentage was observed for NMTS samples (78.6%). In average, saliva samples showed higher Ct values for all tested target genes, compared to those from NPS and NMTS samples. Conclusions: By using the Allplex(TM) 2019-nCoV Assay Kit, saliva samples showed lower sensitivity for SARS CoV-2 compared to NPS samples; however, the not detected cases had lower viral burden in NPS samples (CT values >33) representing an interesting alternative sampling method in patients in which it is not possible to take a NPS sample.
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