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10.1016/j.watres.2021.117002 http://scihub22266oqcxt.onion/10.1016/j.watres.2021.117002 33714910!7927590!33714910     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Water+Res 2021 ; 195 (ä): 117002 Nephropedia Template TP {{tp|p=33714910|t=2021. Viability of SARS-CoV-2 in river water and wastewater at different temperatures and solids content |pdf=|usr=}}{{33714910}} gab.com Text Viability of SARS-CoV-2 in river water and wastewater at different temperatures and solids content JO: Water Res http://www.kidney.de/pget.php?&tw=1&pmid=33714910 #FOAvip COVID-19 patients can excrete viable SARS-CoV-2 virus via urine and faeces, which has raised concerns over the possibility of COVID-19 transmission via aerosolized contaminated water or via the faecal-oral route. These concerns are especially exacerbated in many low- and middle-income countries, where untreated sewage is frequently discharged to surface waters. SARS-CoV-2 RNA has been detected in river water (RW) and raw wastewater (WW) samples. However, little is known about SARS-CoV-2 viability in these environmental matrices. Determining the persistence of SARS-CoV-2 in water under different environmental conditions is of great importance for basic assumptions in quantitative microbial risk assessment (QMRA). In this study, the persistence of SARS-CoV-2 was assessed using plaque assays following spiking of RW and WW samples with infectious SARS-CoV-2 that was previously isolated from a COVID-19 patient. These assays were carried out on autoclaved RW and WW samples, filtered (0.22 microm) and unfiltered, at 4 degrees C and 24 degrees C. Linear and nonlinear regression models were adjusted to the data. The Weibull regression model achieved the lowest root mean square error (RMSE) and was hence chosen to estimate T(90) and T(99) (time required for 1 log and 2 log reductions, respectively). SARS-CoV-2 remained viable longer in filtered compared with unfiltered samples. RW and WW showed T(90) values of 1.9 and 1.2 day and T(99) values of 6.4 and 4.0 days, respectively. When samples were filtered through 0.22 microm pore size membranes, T(90) values increased to 3.3 and 1.5 days, and T(99) increased to 8.5 and 4.5 days, for RW and WW samples, respectively. Remarkable increases in SARS-CoV-2 persistence were observed in assays at 4 degrees C, which showed T(90) values of 7.7 and 5.5 days, and T(99) values of 18.7 and 17.5 days for RW and WW, respectively. These results highlight the variability of SARS-CoV-2 persistence in water and wastewater matrices and can be highly relevant to efforts aimed at quantifying water-related risks, which could be valuable for understanding and controlling the pandemic. Twit Text FOAVip Viability of SARS-CoV-2 in river water and wastewater at different temperatures and solids content ????Water Res http://www.kidney.de/pget.php?&tw=1&pmid=33714910 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33714910 #FOAvip English Wikipedia <ref>{{Cite pmid|33714910}}</ref> Viability of SARS-CoV-2 in river water and wastewater at different temperatures and solids content #MMPMID33714910 de Oliveira LC; Torres-Franco AF; Lopes BC; Santos BSADS; Costa EA; Costa MS; Reis MTP; Melo MC; Polizzi RB; Teixeira MM; Mota CR Water Res 2021[May]; 195 (ä): 117002 PMID33714910show ga COVID-19 patients can excrete viable SARS-CoV-2 virus via urine and faeces, which has raised concerns over the possibility of COVID-19 transmission via aerosolized contaminated water or via the faecal-oral route. These concerns are especially exacerbated in many low- and middle-income countries, where untreated sewage is frequently discharged to surface waters. SARS-CoV-2 RNA has been detected in river water (RW) and raw wastewater (WW) samples. However, little is known about SARS-CoV-2 viability in these environmental matrices. Determining the persistence of SARS-CoV-2 in water under different environmental conditions is of great importance for basic assumptions in quantitative microbial risk assessment (QMRA). In this study, the persistence of SARS-CoV-2 was assessed using plaque assays following spiking of RW and WW samples with infectious SARS-CoV-2 that was previously isolated from a COVID-19 patient. These assays were carried out on autoclaved RW and WW samples, filtered (0.22 microm) and unfiltered, at 4 degrees C and 24 degrees C. Linear and nonlinear regression models were adjusted to the data. The Weibull regression model achieved the lowest root mean square error (RMSE) and was hence chosen to estimate T(90) and T(99) (time required for 1 log and 2 log reductions, respectively). SARS-CoV-2 remained viable longer in filtered compared with unfiltered samples. RW and WW showed T(90) values of 1.9 and 1.2 day and T(99) values of 6.4 and 4.0 days, respectively. When samples were filtered through 0.22 microm pore size membranes, T(90) values increased to 3.3 and 1.5 days, and T(99) increased to 8.5 and 4.5 days, for RW and WW samples, respectively. Remarkable increases in SARS-CoV-2 persistence were observed in assays at 4 degrees C, which showed T(90) values of 7.7 and 5.5 days, and T(99) values of 18.7 and 17.5 days for RW and WW, respectively. These results highlight the variability of SARS-CoV-2 persistence in water and wastewater matrices and can be highly relevant to efforts aimed at quantifying water-related risks, which could be valuable for understanding and controlling the pandemic. |*COVID-19[MESH] |*Wastewater[MESH] |Humans[MESH] |RNA, Viral[MESH] |Rivers[MESH] |SARS-CoV-2[MESH] |Temperature[MESH]Viability of SARS-CoV-2 in river water and wastewater at different tem(epmc).pdf DeepDyve Pubget Overpricing