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10.1016/j.scitotenv.2021.146198 http://scihub22266oqcxt.onion/10.1016/j.scitotenv.2021.146198 33714813!7938741!33714813     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33714813&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Total+Environ 2021 ; 778 (ä): 146198 Nephropedia Template TP {{tp|p=33714813|t=2021. The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 |pdf=|usr=}}{{33714813}} gab.com Text The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 JO: Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=33714813 #FOAvip Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 +/- 0.02 log(10) SARS-CoV-2 genome copies (GC) L(-1)), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 +/- 0.12 log(10) SARS-CoV-2 GC L(-1), ranging from 5.49 +/- 0.02 log(10) GC L(-1) (27th November 2019) to 6.68 +/- 0.02 log(10) GC L(-1) (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). Twit Text FOAVip The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 ????Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=33714813 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33714813 #FOAvip English Wikipedia <ref>{{Cite pmid|33714813}}</ref> The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 #MMPMID33714813 Fongaro G; Stoco PH; Souza DSM; Grisard EC; Magri ME; Rogovski P; Schorner MA; Barazzetti FH; Christoff AP; de Oliveira LFV; Bazzo ML; Wagner G; Hernandez M; Rodriguez-Lazaro D Sci Total Environ 2021[Jul]; 778 (ä): 146198 PMID33714813show ga Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 +/- 0.02 log(10) SARS-CoV-2 genome copies (GC) L(-1)), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 +/- 0.12 log(10) SARS-CoV-2 GC L(-1), ranging from 5.49 +/- 0.02 log(10) GC L(-1) (27th November 2019) to 6.68 +/- 0.02 log(10) GC L(-1) (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). |*COVID-19[MESH] |*RNA, Viral[MESH] |Brazil[MESH] |Humans[MESH] |SARS-CoV-2[MESH]The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Braz(epmc).pdf DeepDyve Pubget Overpricing