Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.virol.2021.01.003 http://scihub22266oqcxt.onion/10.1016/j.virol.2021.01.003 33714753!7849420!33714753     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 2021 ; 558 (ä): 28-37 Nephropedia Template TP {{tp|p=33714753|t=2021. Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody |pdf=|usr=}}{{33714753}} gab.com Text Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=33714753 #FOAvip To help fight COVID-19, new molecular tools specifically targeting critical components of the causative agent of COVID-19, SARS-Coronavirus-2 (SARS-CoV-2), are desperately needed. The SARS-CoV-2 nucleocapsid protein is critical for viral replication, integral to viral particle assembly, and a major diagnostic marker for infection and immune protection. Currently the limited available antibody reagents targeting the nucleocapsid protein are not specific to SARS-CoV-2 nucleocapsid protein, and sequences for these antibodies are not publicly available. In this work we developed and characterized a series of new mouse monoclonal antibodies against the SARS-CoV-2 nucleocapsid protein, with a specific clone, mBG86, targeting only SARS-CoV-2 nucleocapsid protein. The monoclonal antibodies were validated in ELISA, Western blot, and immunofluorescence analyses. The variable regions from six select clones were cloned and sequenced, and preliminary epitope mapping of the sequenced clones was performed. Overall, these new antibody reagents will be of significant value in the fight against COVID-19. Twit Text FOAVip Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=33714753 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33714753 #FOAvip English Wikipedia <ref>{{Cite pmid|33714753}}</ref> Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody #MMPMID33714753 Terry JS; Anderson LB; Scherman MS; McAlister CE; Perera R; Schountz T; Geiss BJ Virology 2021[Jun]; 558 (ä): 28-37 PMID33714753show ga To help fight COVID-19, new molecular tools specifically targeting critical components of the causative agent of COVID-19, SARS-Coronavirus-2 (SARS-CoV-2), are desperately needed. The SARS-CoV-2 nucleocapsid protein is critical for viral replication, integral to viral particle assembly, and a major diagnostic marker for infection and immune protection. Currently the limited available antibody reagents targeting the nucleocapsid protein are not specific to SARS-CoV-2 nucleocapsid protein, and sequences for these antibodies are not publicly available. In this work we developed and characterized a series of new mouse monoclonal antibodies against the SARS-CoV-2 nucleocapsid protein, with a specific clone, mBG86, targeting only SARS-CoV-2 nucleocapsid protein. The monoclonal antibodies were validated in ELISA, Western blot, and immunofluorescence analyses. The variable regions from six select clones were cloned and sequenced, and preliminary epitope mapping of the sequenced clones was performed. Overall, these new antibody reagents will be of significant value in the fight against COVID-19. |*Antibodies, Monoclonal/immunology/isolation & purification[MESH] |*Antibodies, Viral/immunology/isolation & purification[MESH] |Animals[MESH] |COVID-19/epidemiology/*immunology[MESH] |Cloning, Molecular[MESH] |Coronavirus Nucleocapsid Proteins/*immunology[MESH] |Escherichia coli[MESH] |Female[MESH] |Humans[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Phosphoproteins/immunology[MESH] |Recombinant Proteins/immunology[MESH]Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody (epmc).pdf DeepDyve Pubget Overpricing