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10.1016/j.talanta.2021.122154 http://scihub22266oqcxt.onion/10.1016/j.talanta.2021.122154 33714462!7875706!33714462     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Talanta 2021 ; 227 (ä): 122154 Nephropedia Template TP {{tp|p=33714462|t=2021. Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV |pdf=|usr=}}{{33714462}} gab.com Text Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV JO: Talanta http://www.kidney.de/pget.php?&tw=1&pmid=33714462 #FOAvip Infectious diseases caused by viruses such as SARS-CoV-2 and HPV have greatly endangered human health. The nucleic acid detection is essential for the early diagnosis of diseases. Here, we propose a method called PLCR (PfAgo coupled with modified Ligase Chain Reaction for nucleic acid detection) which utilizes PfAgo to only use DNA guides longer than 14-mer to specifically cleave DNA and LCR to precisely distinguish single-base mismatch. PLCR can detect DNA or RNA without PCR at attomolar sensitivities, distinguish single base mutation between the genome of wild type SARS-CoV-2 and its mutant spike D614G, effectively distinguish the novel coronavirus from other coronaviruses and finally achieve multiplexed detection in 70 min. Additionally, LCR products can be directly used as DNA guides without additional input guides to simplify primer design. With desirable sensitivity, specificity and simplicity, the method can be extended for detecting other pathogenic microorganisms. Twit Text FOAVip Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV ????Talanta http://www.kidney.de/pget.php?&tw=1&pmid=33714462 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33714462 #FOAvip English Wikipedia <ref>{{Cite pmid|33714462}}</ref> Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV #MMPMID33714462 Wang L; He R; Lv B; Yu X; Liu Y; Yang J; Li W; Wang Y; Zhang H; Yan G; Mao W; Liu L; Wang F; Ma L Talanta 2021[May]; 227 (ä): 122154 PMID33714462show ga Infectious diseases caused by viruses such as SARS-CoV-2 and HPV have greatly endangered human health. The nucleic acid detection is essential for the early diagnosis of diseases. Here, we propose a method called PLCR (PfAgo coupled with modified Ligase Chain Reaction for nucleic acid detection) which utilizes PfAgo to only use DNA guides longer than 14-mer to specifically cleave DNA and LCR to precisely distinguish single-base mismatch. PLCR can detect DNA or RNA without PCR at attomolar sensitivities, distinguish single base mutation between the genome of wild type SARS-CoV-2 and its mutant spike D614G, effectively distinguish the novel coronavirus from other coronaviruses and finally achieve multiplexed detection in 70 min. Additionally, LCR products can be directly used as DNA guides without additional input guides to simplify primer design. With desirable sensitivity, specificity and simplicity, the method can be extended for detecting other pathogenic microorganisms. |Alphapapillomavirus/chemistry/isolation & purification[MESH] |Argonaute Proteins/*chemistry[MESH] |COVID-19/diagnosis[MESH] |DNA, Viral/*analysis/chemistry[MESH] |Humans[MESH] |Ligase Chain Reaction/*methods[MESH] |Limit of Detection[MESH] |Mutation[MESH] |Papillomavirus Infections/diagnosis[MESH] |Pyrococcus furiosus/*enzymology[MESH] |RNA, Viral/*analysis/chemistry[MESH] |SARS-CoV-2/chemistry/isolation & purification[MESH] |Sensitivity and Specificity[MESH]Pyrococcus furiosus Argonaute coupled with modified ligase chain react(epmc).pdf DeepDyve Pubget Overpricing