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10.1016/j.molcel.2021.02.036 http://scihub22266oqcxt.onion/10.1016/j.molcel.2021.02.036 33713597!7927579!33713597     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell 2021 ; 81 (10): 2135-2147.e5 Nephropedia Template TP {{tp|p=33713597|t=2021. The SARS-CoV-2 subgenome landscape and its novel regulatory features |pdf=|usr=}}{{33713597}} gab.com Text The SARS-CoV-2 subgenome landscape and its novel regulatory features JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=33713597 #FOAvip Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently a global pandemic. CoVs are known to generate negative subgenomes (subgenomic RNAs [sgRNAs]) through transcription-regulating sequence (TRS)-dependent template switching, but the global dynamic landscapes of coronaviral subgenomes and regulatory rules remain unclear. Here, using next-generation sequencing (NGS) short-read and Nanopore long-read poly(A) RNA sequencing in two cell types at multiple time points after infection with SARS-CoV-2, we identified hundreds of template switches and constructed the dynamic landscapes of SARS-CoV-2 subgenomes. Interestingly, template switching could occur in a bidirectional manner, with diverse SARS-CoV-2 subgenomes generated from successive template-switching events. The majority of template switches result from RNA-RNA interactions, including seed and compensatory modes, with terminal pairing status as a key determinant. Two TRS-independent template switch modes are also responsible for subgenome biogenesis. Our findings reveal the subgenome landscape of SARS-CoV-2 and its regulatory features, providing a molecular basis for understanding subgenome biogenesis and developing novel anti-viral strategies. Twit Text FOAVip The SARS-CoV-2 subgenome landscape and its novel regulatory features ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=33713597 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33713597 #FOAvip English Wikipedia <ref>{{Cite pmid|33713597}}</ref> The SARS-CoV-2 subgenome landscape and its novel regulatory features #MMPMID33713597 Wang D; Jiang A; Feng J; Li G; Guo D; Sajid M; Wu K; Zhang Q; Ponty Y; Will S; Liu F; Yu X; Li S; Liu Q; Yang XL; Guo M; Li X; Chen M; Shi ZL; Lan K; Chen Y; Zhou Y Mol Cell 2021[May]; 81 (10): 2135-2147.e5 PMID33713597show ga Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently a global pandemic. CoVs are known to generate negative subgenomes (subgenomic RNAs [sgRNAs]) through transcription-regulating sequence (TRS)-dependent template switching, but the global dynamic landscapes of coronaviral subgenomes and regulatory rules remain unclear. Here, using next-generation sequencing (NGS) short-read and Nanopore long-read poly(A) RNA sequencing in two cell types at multiple time points after infection with SARS-CoV-2, we identified hundreds of template switches and constructed the dynamic landscapes of SARS-CoV-2 subgenomes. Interestingly, template switching could occur in a bidirectional manner, with diverse SARS-CoV-2 subgenomes generated from successive template-switching events. The majority of template switches result from RNA-RNA interactions, including seed and compensatory modes, with terminal pairing status as a key determinant. Two TRS-independent template switch modes are also responsible for subgenome biogenesis. Our findings reveal the subgenome landscape of SARS-CoV-2 and its regulatory features, providing a molecular basis for understanding subgenome biogenesis and developing novel anti-viral strategies. |*COVID-19/genetics/metabolism[MESH] |*Genome, Viral[MESH] |*High-Throughput Nucleotide Sequencing[MESH] |*RNA, Viral/genetics/metabolism[MESH] |*SARS-CoV-2/genetics/metabolism[MESH] |Animals[MESH] |Caco-2 Cells[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH]The SARS-CoV-2 subgenome landscape and its novel regulatory features (epmc).pdf DeepDyve Pubget Overpricing