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10.1016/j.virol.2021.02.010 http://scihub22266oqcxt.onion/10.1016/j.virol.2021.02.010 33711560!7934794!33711560     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 2021 ; 558 (ä): 22-27 Nephropedia Template TP {{tp|p=33711560|t=2021. SARS_CoV2 RBD gene transcription cannot be driven by CMV promoter |pdf=|usr=}}{{33711560}} gab.com Text SARS_CoV2 RBD gene transcription cannot be driven by CMV promoter JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=33711560 #FOAvip Cytomegalovirus (CMV) promoter drives various gene expression and yields sufficient protein for further functional investigation. Receptor binding domain (RBD) on spike protein of the SARS_CoV2 is the most critical portal for virus infection. Thus native conformational RBD protein may facilitate biochemical identification of RBD and provide valuable support of drug and vaccine design for curing COVID-19. We attempted to express RBD under CMV promoter in vitro, but failed. RBD-specific mRNA cannot be detected in cell transfected with recombinant plasmids, in which CMV promoter governs the RBD transcription. Additionally, the pCMV-Tag2B-SARS_CoV2_RBD trans-inactivates CMV promoter transcription activity. Alternatively, we identified that both Chicken beta-actin promoter and Vaccinia virus-specific medium/late (M/L) promoter (pSYN) can highly precede SARS_CoV2 RBD expression. Our findings provided evidence that SARS_CoV2 RBD gene can be driven by Chicken beta-actin promoter or Vaccinia virus-specific medium/late promoter instead of CMV promoter, thus providing valuable information for RBD feature exploration. Twit Text FOAVip SARS_CoV2 RBD gene transcription cannot be driven by CMV promoter ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=33711560 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33711560 #FOAvip English Wikipedia <ref>{{Cite pmid|33711560}}</ref> SARS_CoV2 RBD gene transcription cannot be driven by CMV promoter #MMPMID33711560 Xie L; Yi K; Li Y Virology 2021[Jun]; 558 (ä): 22-27 PMID33711560show ga Cytomegalovirus (CMV) promoter drives various gene expression and yields sufficient protein for further functional investigation. Receptor binding domain (RBD) on spike protein of the SARS_CoV2 is the most critical portal for virus infection. Thus native conformational RBD protein may facilitate biochemical identification of RBD and provide valuable support of drug and vaccine design for curing COVID-19. We attempted to express RBD under CMV promoter in vitro, but failed. RBD-specific mRNA cannot be detected in cell transfected with recombinant plasmids, in which CMV promoter governs the RBD transcription. Additionally, the pCMV-Tag2B-SARS_CoV2_RBD trans-inactivates CMV promoter transcription activity. Alternatively, we identified that both Chicken beta-actin promoter and Vaccinia virus-specific medium/late (M/L) promoter (pSYN) can highly precede SARS_CoV2 RBD expression. Our findings provided evidence that SARS_CoV2 RBD gene can be driven by Chicken beta-actin promoter or Vaccinia virus-specific medium/late promoter instead of CMV promoter, thus providing valuable information for RBD feature exploration. |*Promoter Regions, Genetic[MESH] |Actins/genetics[MESH] |Animals[MESH] |CHO Cells[MESH] |COVID-19/*virology[MESH] |Chickens[MESH] |Chlorocebus aethiops[MESH] |Cloning, Molecular[MESH] |Cricetulus[MESH] |Cytomegalovirus/*genetics[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Protein Domains[MESH] |SARS-CoV-2/*genetics[MESH] |Spike Glycoprotein, Coronavirus/*genetics[MESH] |Transcription, Genetic[MESH] |Vaccinia/genetics[MESH]SARS_CoV2 RBD gene transcription cannot be driven by CMV promoter (epmc).pdf DeepDyve Pubget Overpricing