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10.1016/j.jmoldx.2021.02.009 http://scihub22266oqcxt.onion/10.1016/j.jmoldx.2021.02.009 33706009!7939975!33706009     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33706009&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Mol+Diagn 2021 ; 23 (6): 683-690 Nephropedia Template TP {{tp|p=33706009|t=2021. Microchip RT-PCR Detection of Nasopharyngeal SARS-CoV-2 Samples |pdf=|usr=}}{{33706009}} gab.com Text Microchip RT-PCR Detection of Nasopharyngeal SARS-CoV-2 Samples JO: J Mol Diagn http://www.kidney.de/pget.php?&tw=1&pmid=33706009 #FOAvip Fast, accurate, and reliable diagnostic tests are critical for controlling the spread of the coronavirus disease 2019 (COVID-19) associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The current gold standard for testing is real-time PCR; however, during the current pandemic, supplies of testing kits and reagents have been limited. We report the validation of a rapid (30 minutes), user-friendly, and accurate microchip real-time PCR assay for detection of SARS-CoV-2 from nasopharyngeal swab RNA extracts. Microchips preloaded with COVID-19 primers and probes for the N gene accommodate 1.2-muL reaction volumes, lowering the required reagents by 10-fold compared with tube-based real-time PCR. We validated our assay using contrived reference samples and 21 clinical samples from patients in Canada, determining a limit of detection of 1 copy per reaction. The microchip real-time PCR provides a significantly lower resource alternative to the Centers for Disease Control and Prevention-approved real-time RT-PCR assays with comparable sensitivity, showing 100% positive and negative predictive agreement of clinical samples. Twit Text FOAVip Microchip RT-PCR Detection of Nasopharyngeal SARS-CoV-2 Samples ????J Mol Diagn http://www.kidney.de/pget.php?&tw=1&pmid=33706009 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33706009 #FOAvip English Wikipedia <ref>{{Cite pmid|33706009}}</ref> Microchip RT-PCR Detection of Nasopharyngeal SARS-CoV-2 Samples #MMPMID33706009 Cojocaru R; Yaseen I; Unrau PJ; Lowe CF; Ritchie G; Romney MG; Sin DD; Gill S; Slyadnev M J Mol Diagn 2021[Jun]; 23 (6): 683-690 PMID33706009show ga Fast, accurate, and reliable diagnostic tests are critical for controlling the spread of the coronavirus disease 2019 (COVID-19) associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The current gold standard for testing is real-time PCR; however, during the current pandemic, supplies of testing kits and reagents have been limited. We report the validation of a rapid (30 minutes), user-friendly, and accurate microchip real-time PCR assay for detection of SARS-CoV-2 from nasopharyngeal swab RNA extracts. Microchips preloaded with COVID-19 primers and probes for the N gene accommodate 1.2-muL reaction volumes, lowering the required reagents by 10-fold compared with tube-based real-time PCR. We validated our assay using contrived reference samples and 21 clinical samples from patients in Canada, determining a limit of detection of 1 copy per reaction. The microchip real-time PCR provides a significantly lower resource alternative to the Centers for Disease Control and Prevention-approved real-time RT-PCR assays with comparable sensitivity, showing 100% positive and negative predictive agreement of clinical samples. |*Lab-On-A-Chip Devices[MESH] |Benchmarking[MESH] |COVID-19 Nucleic Acid Testing/instrumentation/methods/*standards[MESH] |COVID-19/*diagnosis/epidemiology[MESH] |Canada/epidemiology[MESH] |Humans[MESH] |Limit of Detection[MESH] |Nasopharynx/virology[MESH] |Point-of-Care Testing[MESH] |RNA, Viral/*genetics[MESH] |Reagent Kits, Diagnostic/supply & distribution[MESH] |Real-Time Polymerase Chain Reaction/*standards[MESH]Microchip RT-PCR Detection of Nasopharyngeal SARS-CoV-2 Samples (epmc).pdf DeepDyve Pubget Overpricing