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10.1016/j.actbio.2021.03.007 http://scihub22266oqcxt.onion/10.1016/j.actbio.2021.03.007 33705988!8096718!33705988     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Acta+Biomater 2021 ; 126 (ä): 144-153 Nephropedia Template TP {{tp|p=33705988|t=2021. Loss of sarcomeric proteins via upregulation of JAK/STAT signaling underlies interferon-gamma-induced contractile deficit in engineered human myocardium |pdf=|usr=}}{{33705988}} gab.com Text Loss of sarcomeric proteins via upregulation of JAK/STAT signaling underlies interferon-gamma-induced contractile deficit in engineered human myocardium JO: Acta Biomater http://www.kidney.de/pget.php?&tw=1&pmid=33705988 #FOAvip The level of circulating interferon-gamma (IFNgamma) is elevated in various clinical conditions including autoimmune and inflammatory diseases, sepsis, acute coronary syndrome, and viral infections. As these conditions are associated with high risk of myocardial dysfunction, we investigated the effects of IFNgamma on 3D fibrin-based engineered human cardiac tissues ("cardiobundles"). Cardiobundles were fabricated from human pluripotent stem cell-derived cardiomyocytes, exposed to 0-20 ng/ml of IFNgamma on culture days 7-14, and assessed for changes in tissue structure, viability, contractile force and calcium transient generation, action potential propagation, cytokine secretion, and expression of select genes and proteins. We found that application of IFNgamma induced a dose-dependent reduction in contractile force generation, deterioration of sarcomeric organization, and cardiomyocyte disarray, without significantly altering cell viability, action potential propagation, or calcium transient amplitude. At molecular level, the IFNgamma-induced structural and functional deficits could be attributed to altered balance of pro- and anti-inflammatory cytokines, upregulation of JAK/STAT signaling pathway (JAK1, JAK2, and STAT1), and reduced expression of myosin heavy chain, myosin light chain-2v, and sarcomeric alpha-actinin. Application of clinically used JAK/STAT inhibitors, tofacitinib and baricitinib, fully prevented IFNgamma-induced cardiomyopathy, confirming the critical roles of this signaling pathway in inflammatory cardiac disease. Taken together, our in vitro studies in engineered myocardial tissues reveal direct adverse effects of pro-inflammatory cytokine IFNgamma on human cardiomyocytes and establish the foundation for a potential use of cardiobundle platform in modeling of inflammatory myocardial disease and therapy. STATEMENT OF SIGNIFICANCE: Various inflammatory and autoimmune diseases including rheumatoid arthritis, sepsis, lupus erythematosus, Chagas disease, and others, as well as viral infections including H1N1 influenza and COVID-19 show increased systemic levels of a pro-inflammatory cytokine interferon-gamma (IFNgamma) and are associated with high risk of heart disease. Here we explored for the first time if chronically elevated levels of IFNgamma can negatively affect structure and function of engineered human heart tissues in vitro. Our studies revealed IFNgamma-induced deterioration of myofibrillar organization and contractile force production in human cardiomyocytes, attributed to decreased expression of multiple sarcomeric proteins and upregulation of JAK/STAT signaling pathway. FDA-approved JAK inhibitors fully blocked the adverse effects of IFNgamma, suggesting a potentially effective strategy against human inflammatory cardiomyopathy. Twit Text FOAVip Loss of sarcomeric proteins via upregulation of JAK/STAT signaling underlies interferon-gamma-induced contractile deficit in engineered human myocardium ????Acta Biomater http://www.kidney.de/pget.php?&tw=1&pmid=33705988 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33705988 #FOAvip English Wikipedia <ref>{{Cite pmid|33705988}}</ref> Loss of sarcomeric proteins via upregulation of JAK/STAT signaling underlies interferon-gamma-induced contractile deficit in engineered human myocardium #MMPMID33705988 Zhan RZ; Rao L; Chen Z; Strash N; Bursac N Acta Biomater 2021[May]; 126 (ä): 144-153 PMID33705988show ga The level of circulating interferon-gamma (IFNgamma) is elevated in various clinical conditions including autoimmune and inflammatory diseases, sepsis, acute coronary syndrome, and viral infections. As these conditions are associated with high risk of myocardial dysfunction, we investigated the effects of IFNgamma on 3D fibrin-based engineered human cardiac tissues ("cardiobundles"). Cardiobundles were fabricated from human pluripotent stem cell-derived cardiomyocytes, exposed to 0-20 ng/ml of IFNgamma on culture days 7-14, and assessed for changes in tissue structure, viability, contractile force and calcium transient generation, action potential propagation, cytokine secretion, and expression of select genes and proteins. We found that application of IFNgamma induced a dose-dependent reduction in contractile force generation, deterioration of sarcomeric organization, and cardiomyocyte disarray, without significantly altering cell viability, action potential propagation, or calcium transient amplitude. At molecular level, the IFNgamma-induced structural and functional deficits could be attributed to altered balance of pro- and anti-inflammatory cytokines, upregulation of JAK/STAT signaling pathway (JAK1, JAK2, and STAT1), and reduced expression of myosin heavy chain, myosin light chain-2v, and sarcomeric alpha-actinin. Application of clinically used JAK/STAT inhibitors, tofacitinib and baricitinib, fully prevented IFNgamma-induced cardiomyopathy, confirming the critical roles of this signaling pathway in inflammatory cardiac disease. Taken together, our in vitro studies in engineered myocardial tissues reveal direct adverse effects of pro-inflammatory cytokine IFNgamma on human cardiomyocytes and establish the foundation for a potential use of cardiobundle platform in modeling of inflammatory myocardial disease and therapy. STATEMENT OF SIGNIFICANCE: Various inflammatory and autoimmune diseases including rheumatoid arthritis, sepsis, lupus erythematosus, Chagas disease, and others, as well as viral infections including H1N1 influenza and COVID-19 show increased systemic levels of a pro-inflammatory cytokine interferon-gamma (IFNgamma) and are associated with high risk of heart disease. Here we explored for the first time if chronically elevated levels of IFNgamma can negatively affect structure and function of engineered human heart tissues in vitro. Our studies revealed IFNgamma-induced deterioration of myofibrillar organization and contractile force production in human cardiomyocytes, attributed to decreased expression of multiple sarcomeric proteins and upregulation of JAK/STAT signaling pathway. FDA-approved JAK inhibitors fully blocked the adverse effects of IFNgamma, suggesting a potentially effective strategy against human inflammatory cardiomyopathy. |*COVID-19[MESH] |*Influenza A Virus, H1N1 Subtype[MESH] |Humans[MESH] |Interferon-gamma/pharmacology[MESH] |Myocardium[MESH] |SARS-CoV-2[MESH] |Signal Transduction[MESH]Loss of sarcomeric proteins via upregulation of JAK/STAT signaling und(epmc).pdf DeepDyve Pubget Overpricing