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10.1093/jalm/jfab014

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33693808!7989439!33693808
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suck abstract from ncbi


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pmid33693808      J+Appl+Lab+Med 2021 ; 6 (4): 942-952
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  • Performance of 4 Automated SARS-CoV-2 Serology Assay Platforms in a Large Cohort Including Susceptible COVID-19-Negative and COVID-19-Positive Patients #MMPMID33693808
  • Ward MD; Mullins KE; Pickett E; Merrill V; Ruiz M; Rebuck H; Duh SH; Christenson RH
  • J Appl Lab Med 2021[Jul]; 6 (4): 942-952 PMID33693808show ga
  • BACKGROUND: Anti-SARS-CoV-2 serological responses may have a vital role in controlling the spread of the disease. However, the comparative performance of automated serological assays has not been determined in susceptible patients with significant comorbidities. METHODS: In this study, we used large numbers of samples from patients who were negative (n = 2030) or positive (n = 112) for COVID-19 to compare the performance of 4 serological assay platforms: Siemens Healthineers Atellica IM Analyzer, Siemens Healthineers Dimension EXL Systems, Abbott ARCHITECT, and Roche cobas. RESULTS: All 4 serology assay platforms exhibited comparable negative percentage of agreement with negative COVID-19 status ranging from 99.2% to 99.7% and positive percentage of agreement from 84.8% to 87.5% with positive real-time reverse transcriptase PCR results. Of the 2142 total samples, only 38 samples (1.8%) yielded discordant results on one or more platforms. However, only 1.1% (23/2030) of results from the COVID-19-negative cohort were discordant. whereas discordance was 10-fold higher for the COVID-19-positive cohort, at 11.3% (15/112). Of the total 38 discordant results, 34 were discordant on only one platform. CONCLUSIONS: Serology assay performance was comparable across the 4 platforms assessed in a large population of patients who were COVID-19 negative with relevant comorbidities. The pattern of discordance shows that samples were discordant on a single assay platform, and the discordance rate was 10-fold higher in the population that was COVID-19 positive.
  • |*COVID-19 Serological Testing[MESH]
  • |Aged[MESH]
  • |Antibodies, Viral/*blood/immunology[MESH]
  • |Biological Assay/methods/*statistics & numerical data[MESH]
  • |COVID-19/blood/*diagnosis/epidemiology/virology[MESH]
  • |Cohort Studies[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |SARS-CoV-2/*immunology[MESH]


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