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10.1186/s12985-021-01525-z http://scihub22266oqcxt.onion/10.1186/s12985-021-01525-z 33691737!7944464!33691737     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virol+J 2021 ; 18 (1): 53 Nephropedia Template TP {{tp|p=33691737|t=2021. Optimal preparation of SARS-CoV-2 viral transport medium for culture |pdf=|usr=}}{{33691737}} gab.com Text Optimal preparation of SARS-CoV-2 viral transport medium for culture JO: Virol J http://www.kidney.de/pget.php?&tw=1&pmid=33691737 #FOAvip INTRODUCTION: The sudden arrival of the COVID-19 pandemic placed significant stresses on supply chains including viral transport medium (VTM). The VTM that was urgently required needed to support viral replication, as well as other routine diagnostic approaches. We describe the preparation and validation testing of VTM for rapidly expanding diagnostic testing, where the capacity of the VTM to preserve viral integrity, for culture, isolation and full sequence analysis, was maintained. METHODS: VTM was prepared using different methods of sterilization then 'spiked' with virus. The VTM was investigated using viral culture in Vero cells, and for nucleic acid detection by quantitative PCR. RESULTS: The best results were obtained by filter and autoclave-based sterilization. The VTM proved robust for culture-based analyses provided the inoculated VTM was stored at 4 degrees C, and tested within 48 h. The filtered VTM also supported PCR-based diagnosis for at least 5 days when the mock inoculated VTM was held at room temperature. DISCUSSION: The manual handling of VTM production, including filling and sterilization, was optimized. SARS-CoV-2 was spiked into VTM to assess different sterilization methods and measure the effects of storage time and temperature upon VTM performance. While most diagnostic protocols will not require replication competent virus, the use of high quality VTM will allow for the next phase of laboratory analysis in the COVID-19 pandemic, including drug and antibody susceptibility analysis of re-isolated SARS-CoV-2, and for the testing of vaccine escape mutants. Twit Text FOAVip Optimal preparation of SARS-CoV-2 viral transport medium for culture ????Virol J http://www.kidney.de/pget.php?&tw=1&pmid=33691737 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33691737 #FOAvip English Wikipedia <ref>{{Cite pmid|33691737}}</ref> Optimal preparation of SARS-CoV-2 viral transport medium for culture #MMPMID33691737 McAuley J; Fraser C; Paraskeva E; Trajcevska E; Sait M; Wang N; Bert E; Purcell D; Strugnell R Virol J 2021[Mar]; 18 (1): 53 PMID33691737show ga INTRODUCTION: The sudden arrival of the COVID-19 pandemic placed significant stresses on supply chains including viral transport medium (VTM). The VTM that was urgently required needed to support viral replication, as well as other routine diagnostic approaches. We describe the preparation and validation testing of VTM for rapidly expanding diagnostic testing, where the capacity of the VTM to preserve viral integrity, for culture, isolation and full sequence analysis, was maintained. METHODS: VTM was prepared using different methods of sterilization then 'spiked' with virus. The VTM was investigated using viral culture in Vero cells, and for nucleic acid detection by quantitative PCR. RESULTS: The best results were obtained by filter and autoclave-based sterilization. The VTM proved robust for culture-based analyses provided the inoculated VTM was stored at 4 degrees C, and tested within 48 h. The filtered VTM also supported PCR-based diagnosis for at least 5 days when the mock inoculated VTM was held at room temperature. DISCUSSION: The manual handling of VTM production, including filling and sterilization, was optimized. SARS-CoV-2 was spiked into VTM to assess different sterilization methods and measure the effects of storage time and temperature upon VTM performance. While most diagnostic protocols will not require replication competent virus, the use of high quality VTM will allow for the next phase of laboratory analysis in the COVID-19 pandemic, including drug and antibody susceptibility analysis of re-isolated SARS-CoV-2, and for the testing of vaccine escape mutants. |Animals[MESH] |Anti-Bacterial Agents/pharmacology[MESH] |COVID-19 Testing/methods[MESH] |COVID-19/*diagnosis[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Culture Media/chemistry[MESH] |Humans[MESH] |RNA, Viral/analysis[MESH] |SARS-CoV-2/*growth & development[MESH] |Specimen Handling/*methods[MESH]Optimal preparation of SARS-CoV-2 viral transport medium for culture (epmc).pdf DeepDyve Pubget Overpricing