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10.1016/j.cell.2021.02.044 http://scihub22266oqcxt.onion/10.1016/j.cell.2021.02.044 33691138!7901297!33691138     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2021 ; 184 (8): 2229-2238.e13 Nephropedia Template TP {{tp|p=33691138|t=2021. A trans-complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence |pdf=|usr=}}{{33691138}} gab.com Text A trans-complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=33691138 #FOAvip The biosafety level 3 (BSL-3) requirement to culture severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a bottleneck for research. Here, we report a trans-complementation system that produces single-round infectious SARS-CoV-2 that recapitulates authentic viral replication. We demonstrate that the single-round infectious SARS-CoV-2 can be used at BSL-2 laboratories for high-throughput neutralization and antiviral testing. The trans-complementation system consists of two components: a genomic viral RNA containing ORF3 and envelope gene deletions, as well as mutated transcriptional regulator sequences, and a producer cell line expressing the two deleted genes. Trans-complementation of the two components generates virions that can infect naive cells for only one round but does not produce wild-type SARS-CoV-2. Hamsters and K18-hACE2 transgenic mice inoculated with the complementation-derived virions exhibited no detectable disease, even after intracranial inoculation with the highest possible dose. Thus, the trans-complementation platform can be safely used at BSL-2 laboratories for research and countermeasure development. Twit Text FOAVip A trans-complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=33691138 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33691138 #FOAvip English Wikipedia <ref>{{Cite pmid|33691138}}</ref> A trans-complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence #MMPMID33691138 Zhang X; Liu Y; Liu J; Bailey AL; Plante KS; Plante JA; Zou J; Xia H; Bopp NE; Aguilar PV; Ren P; Menachery VD; Diamond MS; Weaver SC; Xie X; Shi PY Cell 2021[Apr]; 184 (8): 2229-2238.e13 PMID33691138show ga The biosafety level 3 (BSL-3) requirement to culture severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a bottleneck for research. Here, we report a trans-complementation system that produces single-round infectious SARS-CoV-2 that recapitulates authentic viral replication. We demonstrate that the single-round infectious SARS-CoV-2 can be used at BSL-2 laboratories for high-throughput neutralization and antiviral testing. The trans-complementation system consists of two components: a genomic viral RNA containing ORF3 and envelope gene deletions, as well as mutated transcriptional regulator sequences, and a producer cell line expressing the two deleted genes. Trans-complementation of the two components generates virions that can infect naive cells for only one round but does not produce wild-type SARS-CoV-2. Hamsters and K18-hACE2 transgenic mice inoculated with the complementation-derived virions exhibited no detectable disease, even after intracranial inoculation with the highest possible dose. Thus, the trans-complementation platform can be safely used at BSL-2 laboratories for research and countermeasure development. |*SARS-CoV-2/genetics/pathogenicity/physiology[MESH] |A549 Cells[MESH] |Animals[MESH] |COVID-19/*virology[MESH] |Chlorocebus aethiops[MESH] |Containment of Biohazards/*methods[MESH] |Cricetinae[MESH] |Genetic Complementation Test/methods[MESH] |Genome, Viral[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Male[MESH] |Mice[MESH] |Mice, Transgenic[MESH] |RNA, Viral[MESH] |Vero Cells[MESH] |Virulence[MESH]A trans-complementation system for SARS-CoV-2 recapitulates authentic (epmc).pdf DeepDyve Pubget Overpricing