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10.3346/jkms.2021.36.e64

http://scihub22266oqcxt.onion/10.3346/jkms.2021.36.e64
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suck abstract from ncbi


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pmid33686810      J+Korean+Med+Sci 2021 ; 36 (9): e64
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  • Comparison of Serologic Response of Hospitalized COVID-19 Patients Using 8 Immunoassays #MMPMID33686810
  • Lee SM; Kim IS; Lim S; Lee SJ; Kim WJ; Shin KH; Moon SY; Chang CL
  • J Korean Med Sci 2021[Mar]; 36 (9): e64 PMID33686810show ga
  • BACKGROUND: In Korea, there were issues regarding the use of immunoassays for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies to detect infection. So, we compared antibody results of eight kinds of commercial immunoassays using clinical remnant specimens. METHODS: We compared the results of several immunoassay kits tested on 40 serum samples from 15 confirmed patients and 86 remnant serum samples from clinical laboratory. Eight kinds of IVD kits-four enzyme-linked immunosorbent assay, two lateral flow rapid immunochromatographic assays, and two chemiluminescent immunoassays with one RUO kit were tested. RESULTS: Among 40 serum samples from 15 coronavirus disease 2019 (COVID-19) patients, 35 yielded at least one positive result for detecting antibodies in the combined assessment. There were inconsistent results in 12 (28%) samples by single immunoassay. Forty samples collected in 2019 before the first COVID-19 Korean case showed negative results except for one equivocal result. CONCLUSION: The discrepant results obtained with different immunoassay kits in this study show that serological assessment of SARS-CoV-2 by a single immunoassay requires caution not only in detecting infection but also in assessing immunologic status.
  • |Antibodies, Viral/*blood[MESH]
  • |COVID-19/*diagnosis/virology[MESH]
  • |Hospitalization[MESH]
  • |Humans[MESH]
  • |Immunoassay/*methods[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Reagent Kits, Diagnostic[MESH]


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