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Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Angew+Chem+Int+Ed+Engl 2021 ; 60 (18): 10273-10278 Nephropedia Template TP
Liu X; Wang YL; Wu J; Qi J; Zeng Z; Wan Q; Chen Z; Manandhar P; Cavener VS; Boyle NR; Fu X; Salazar E; Kuchipudi SV; Kapur V; Zhang X; Umetani M; Sen M; Willson RC; Chen SH; Zu Y
Angew Chem Int Ed Engl 2021[Apr]; 60 (18): 10273-10278 PMID33684258show ga
The receptor-binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 spike (S) protein plays a central role in mediating the first step of virus infection to cause disease: virus binding to angiotensin-converting enzyme 2 (ACE2) receptors on human host cells. Therefore, S/RBD is an ideal target for blocking and neutralization therapies to prevent and treat coronavirus disease 2019 (COVID-19). Using a target-based selection approach, we developed oligonucleotide aptamers containing a conserved sequence motif that specifically targets S/RBD. Synthetic aptamers had high binding affinity for S/RBD-coated virus mimics (K(D) approximately 7 nM) and also blocked interaction of S/RBD with ACE2 receptors (IC(50) approximately 5 nM). Importantly, aptamers were able to neutralize S protein-expressing viral particles and prevent host cell infection, suggesting a promising COVID-19 therapy strategy.