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10.3389/fmicb.2021.602754 http://scihub22266oqcxt.onion/10.3389/fmicb.2021.602754 33679631!7933487!33679631     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Microbiol 2021 ; 12 (ä): 602754 Nephropedia Template TP {{tp|p=33679631|t=2021. A Half-Day Genome Sequencing Protocol for Middle East Respiratory Syndrome Coronavirus |pdf=|usr=}}{{33679631}} gab.com Text A Half-Day Genome Sequencing Protocol for Middle East Respiratory Syndrome Coronavirus JO: Front Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33679631 #FOAvip Recent coronavirus (CoV) outbreaks, including that of Middle East respiratory syndrome (MERS), have presented a threat to public health worldwide. A primary concern in these outbreaks is the extent of mutations in the CoV, and the content of viral variation that can be determined only by whole genome sequencing (WGS). We aimed to develop a time efficient WGS protocol, using universal primers spanning the entire MERS-CoV genome. MERS and synthetic Neoromicia capensis bat CoV genomes were successfully amplified using our developed PCR primer set and sequenced with MinION. All experimental and analytical processes took 6 h to complete and were also applied to synthetic animal serum samples, wherein the MERS-CoV genome sequence was completely recovered. Results showed that the complete genome of MERS-CoV and related variants could be directly obtained from clinical samples within half a day. Consequently, this method will contribute to rapid MERS diagnosis, particularly in future CoV epidemics. Twit Text FOAVip A Half-Day Genome Sequencing Protocol for Middle East Respiratory Syndrome Coronavirus ????Front Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33679631 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33679631 #FOAvip English Wikipedia <ref>{{Cite pmid|33679631}}</ref> A Half-Day Genome Sequencing Protocol for Middle East Respiratory Syndrome Coronavirus #MMPMID33679631 Kim KW; Choi S; Shin SK; Lee I; Ku KB; Kim SJ; Kim S; Yi H Front Microbiol 2021[]; 12 (ä): 602754 PMID33679631show ga Recent coronavirus (CoV) outbreaks, including that of Middle East respiratory syndrome (MERS), have presented a threat to public health worldwide. A primary concern in these outbreaks is the extent of mutations in the CoV, and the content of viral variation that can be determined only by whole genome sequencing (WGS). We aimed to develop a time efficient WGS protocol, using universal primers spanning the entire MERS-CoV genome. MERS and synthetic Neoromicia capensis bat CoV genomes were successfully amplified using our developed PCR primer set and sequenced with MinION. All experimental and analytical processes took 6 h to complete and were also applied to synthetic animal serum samples, wherein the MERS-CoV genome sequence was completely recovered. Results showed that the complete genome of MERS-CoV and related variants could be directly obtained from clinical samples within half a day. Consequently, this method will contribute to rapid MERS diagnosis, particularly in future CoV epidemics. äA Half-Day Genome Sequencing Protocol for Middle East Respiratory Synd(epmc).pdf DeepDyve Pubget Overpricing