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10.1016/j.jiac.2021.02.025

http://scihub22266oqcxt.onion/10.1016/j.jiac.2021.02.025
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33676843!7906513!33676843
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suck abstract from ncbi


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pmid33676843      J+Infect+Chemother 2021 ; 27 (6): 915-918
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  • Immunochromatography and chemiluminescent enzyme immunoassay for COVID-19 diagnosis #MMPMID33676843
  • Ishii T; Sasaki M; Yamada K; Kato D; Osuka H; Aoki K; Morita T; Ishii Y; Tateda K
  • J Infect Chemother 2021[Jun]; 27 (6): 915-918 PMID33676843show ga
  • INTRODUCTION: The rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required to prevent the spread of COVID-19. This study evaluated the utility of two SARS-CoV-2 antigen detection methods. METHODS: We evaluated two types of antigen detection methods using immunochromatography (Espline) and quantitative chemiluminescent enzyme immunoassay (Lumipulse). RT-PCR was performed as a standard procedure for COVID-19 diagnosis. Lumipulse and RT-PCR were performed for all 486 nasopharyngeal swabs and 136 saliva samples, and the Espline test was performed for 271 nasopharyngeal swabs and 93 saliva samples. RESULTS: The sensitivity and specificity of the Espline test were 10/11 and 260/260 (100%), respectively for the nasopharyngeal swabs and 3/9 and 84/84 (100%), respectively for the saliva samples. High sensitivities for both saliva (8/9) and nasopharyngeal swabs (22/24) were observed in the Lumipulse test. The specificities of the Lumipulse test for nasopharyngeal swabs and saliva samples were 460/462 (99.6%) and 123/127 (96.9%), respectively. CONCLUSION: The Espline test is not effective for saliva samples but is useful for simple and rapid COVID-19 tests using nasopharyngeal swabs because it does not require special devices. The Lumipulse test is a powerful high-throughput tool for COVID-19 diagnosis because it has high detection performance for nasopharyngeal swabs and saliva samples.
  • |*Chromatography, Affinity[MESH]
  • |*Immunoenzyme Techniques[MESH]
  • |*Luminescent Measurements[MESH]
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Antigens, Viral/isolation & purification[MESH]
  • |COVID-19 Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Child[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Nasopharynx/virology[MESH]
  • |Saliva/virology[MESH]


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